Anti-Lyn (phospho Y507) 抗体 [EP504Y] (ab33914)

製品の概要

  • 製品名
    Anti-Lyn (phospho Y507) antibody [EP504Y]
    Lyn 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP504Y] to Lyn (phospho Y507)
  • 特異性
    The antibody will detect Lyn’s phosphorylation on Tyrosine 507.
  • アプリケーション
    適用あり: IHC-P, WB, IPmore details
    適用なし: Flow Cyt or ICC/IF
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic phospho-peptide corresponding to residues surrounding Tyrosine 507 of Lyn (Human).

  • ポジティブ・コントロール
    • WB: HeLa (untreated and treated with pervanadate) and Raji treated with pervanadate whole cell lysates.
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度
    Tissue culture supernatant
  • ポリ/モノ
    モノクローナル
  • クローン名
    EP504Y
  • アイソタイプ
    IgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab33914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/100.
WB 1/500 - 1/5000. Detects a band of approximately 53-56 kDa (predicted molecular weight: 61 kDa).
IP 1/50.
  • 追加情報
    Is unsuitable for Flow Cyt or ICC/IF.
  • ターゲット情報

    • 機能
      Down regulates expression of stem cell growth factor receptor (KIT). Acts as an effector of EpoR (erythropoietin receptor) in controlling KIT expression and may play a central role in erythroid differentiation during the switch between proliferation and maturation (By similarity). Acts as a positive regulator of cell movement while negatively regulating adhesion to stromal cells by inhibiting the ICAM-1-binding activity of beta-2 integrins. Acts as the mediator that relays suppressing signals from the chemokine receptor CXCR4 to beta-2 integrin LFA-1 in hematopoietic precursors. Involved in induction of stress-activated protein kinase (SAPK), but not ERK or p38 MAPK, in response to genotoxic agents. Induces SAPK by a MKK7- and MEKK1-dependent mechanism. The LYN -> MEKK1 -> MKK7 -> SAPK pathway is functional in the induction of apoptosis by genotoxic agents.
    • 組織特異性
      Widely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary neuroblastoma tumors.
    • 配列類似性
      Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
      Contains 1 protein kinase domain.
      Contains 1 SH2 domain.
      Contains 1 SH3 domain.
    • ドメイン
      The protein kinase domain plays an important role in its localization in the cell membrane.
    • 翻訳後修飾
      Ubiquitinated. Ubiquitination is SH3-dependent.
    • 細胞内局在
      Cell membrane. Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Golgi apparatus. Accumulates in the nucleus by inhibition of CRM1-mediated nuclear export. Nuclear accumulation is increased by inhibition of its kinase activity. The trafficking from the Golgi apparatus to the plasma membrane occurs in a kinase domain-dependent but kinase activity independent manner and is mediated by exocytic vesicular transport.
    • Information by UniProt
    • 参照データベース
    • 製品の状態
      This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), YES1 (P07947), and SRC (P12931). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
    • 別名
      • AA407514 antibody
      • EC 2.7.10.2 antibody
      • FLJ26625 antibody
      • Hck 2 antibody
      • JTK 8 antibody
      • JTK8 antibody
      • Lck/Yes related novel protein tyrosine kinase antibody
      • LYN antibody
      • LYN proto oncogene, Src family tyrosine kinase antibody
      • LYN_HUMAN antibody
      • ONCOGENE LYN antibody
      • p53Lyn antibody
      • p56Lyn antibody
      • Tyrosine protein kinase LYN antibody
      • Tyrosine-protein kinase Lyn antibody
      • V yes 1 Yamaguchi sarcoma viral related oncogene homolog antibody
      • Yamaguchi sarcoma viral (v yes 1) related oncogene homolog antibody
      see all

    画像

    • All lanes : Anti-Lyn (phospho Y507) antibody [EP504Y] (ab33914) at 1/1000 dilution

      Lane 1 : Raji whole cell lysate
      Lane 2 : Raji treated with 1mM pervanadate for 30 min whole cell lysate
      Lane 3 : Raji treated with 1mM pervanadate for 30 min whole cell lysate, the membrane was incubated with alkaline phosphatase.

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 61 kDa
      Observed band size : 61 kDa

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Dot blot analysis of Lyn (phospho Y507) phospho peptide (Lane 1) and Lyn non-phospho peptide (Lane 2) labelling Lyn (phospho Y507) with ab33914 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/100,000.

      Blocking and dilution buffer: 5% NFDM /TBST.

    • All lanes : Anti-Lyn (phospho Y507) antibody [EP504Y] (ab33914) at 1/5000 dilution

      Lane 1 : 10ug Non treated HeLa cell lysate
      Lane 2 : 10ug HeLa cell lysate treated with Pervanadate

      Secondary
      Goat anti-rabbit HRP labeled.

      Predicted band size : 61 kDa

    参考文献

    This product has been referenced in:
    • Sharma N  et al. SHP2 phosphatase promotes mast cell chemotaxis toward stem cell factor via enhancing activation of the Lyn/Vav/Rac signaling axis. J Immunol 192:4859-66 (2014). Read more (PubMed: 24733849) »
    • Luo W  et al. A balance between B cell receptor and inhibitory receptor signaling controls plasma cell differentiation by maintaining optimal Ets1 levels. J Immunol 193:909-20 (2014). Read more (PubMed: 24929000) »

    See all 7 Publications for this product

    レビューと Q&A

    Thank you for your enquiry.

    These 2 antibodies have lot specific concentrations. If you could please forward me the lot numbers for ab40660 and ab33914 I'd be happy to enquire about the concentrations.

    Thanks for the additional info...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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