Anti-Ly76 抗体 [TER-119] (Phycoerythrin) (ab93586)

製品の概要

  • 製品名Anti-Ly76 antibody [TER-119] (Phycoerythrin)
    Ly76 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [TER-119] to Ly76 (Phycoerythrin)
  • 標識Phycoerythrin. Ex: 488nm, Em: 575nm
  • アプリケーション適用あり: Flow Cytmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Tissue/ cell preparation - Mouse fetal liver cells

  • ポジティブ・コントロール
    • BALB/c bone marrow cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab93586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt
  • 追加情報Flow Cyt: Use at a concentration of 0.25 µg/test.
    A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性Ly76 is a glycophorin A-associated protein. It is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not BFU-E or CFU-E.
    • 細胞内局在Plasma membrane
    • 参照データベース
    • 別名
      • Ly 76 antibody
      • Lymphocyte antigen 76 antibody
      • TER 119 antibody
      • TER119 antibody

    Anti-Ly76 antibody [TER-119] (Phycoerythrin) 画像

    • flow cytometric analysis showing staining of BALB/c bone marrow cells with 0.125 µg of PE Rat IgG2b Isotype Control (open histogram) or 0.125 µg of ab93586 (colored histogram).

    Anti-Ly76 antibody [TER-119] (Phycoerythrin) (ab93586) 使用論文

    This product has been referenced in:
    • Kina T  et al. The monoclonal antibody TER-119 recognizes a molecule associated with glycophorin A and specifically marks the late stages of murine erythroid lineage. Br J Haematol 109:280-7 (2000). Read more (PubMed: 10848813) »
    • Vannucchi AM  et al. Identification and characterization of a bipotent (erythroid and megakaryocytic) cell precursor from the spleen of phenylhydrazine-treated mice. Blood 95:2559-68 (2000). Read more (PubMed: 10753835) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Sample Mouse Cell (Erythrocytes)
    Specification Erythrocytes
    Preparation Cell harvesting/tissue preparation method: Isolation of purified erythrocytes from whole blood using percoll gradient
    Sample buffer: PBS with 5mM EDTA
    Fixation Unfixed
    Permeabilization No
    Gating Strategy live cell population of purified erythrocytes gated on the basis of FSC/SSC. P2 gating for positive cell population was set so that <1% of the negative cell population was PE positive.
    Username

    Abcam user community

    Verified customer

    投稿 Mar 25 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"