製品の概要

  • 製品名
    Anti-LY6G5C antibody
  • 製品の詳細
    Rabbit polyclonal to LY6G5C
  • アプリケーション
    適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen from Human LY6G5C

  • ポジティブ・コントロール
    • Human tonsil tissue. RT-4 and U-251 MG lysate. Human Plasma.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab122501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    May have a role in hematopoietic cell differentiation.
  • 組織特異性
    Detected in T-cell lines and fetal and adult lung.
  • 配列類似性
    Contains 1 UPAR/Ly6 domain.
  • 翻訳後修飾
    N-glycosylated.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C6orf20 antibody
    • G5C antibody
    • LY65C_HUMAN antibody
    • LY6G5C antibody
    • LY6G5CA antibody
    • LY6G5CB antibody
    • Lymphocyte antigen 6 complex locus protein G5c antibody
    • NG33 antibody
    see all

画像

  • Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab122501 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-LY6G5C antibody (ab122501) at 1/250 dilution

    Lane 1 : RT-4 lysate
    Lane 2 : U-251 MG lysate
    Lane 3 : Human plasma
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique
  • Immunohistochemical staining of human tonsil using 1/100 ab122501.

参考文献

ab122501 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Baboon Tissue sections (skeletal muscle)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM sodium citrate (pH 6.0)
Permeabilization
No
Specification
skeletal muscle
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Username

Dr. Soon-Ok Kim

Verified customer

投稿 Oct 02 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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