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The details of the immunogen for this antibody are not available.
Our Abpromise guarantee covers the use of ab25024 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells. ab136125-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.|
|IP||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 21-25 kDa.
In vitro complement-dependent cytotoxicity assays: Use at an assay dependent dilution.In vivo depletion of Ly-6G+ cells: Use at an assay dependent dilution.
BALB/c bone marrow cells were stained with either rat IgG2b-FITC (as an isotype control) or rat anti-mouse Ly-6G-FITC (ab25024) at a concentration of 1ug/106 cells. Large cells were then gated and analyzed on a flow cytometer.
Flow Cytometry analysis of BALB/c mouse bone marrow cells labeling Mouse Ly-6G/Ly-6C with ab25024 at 1 μg/106 cells. A Rat Anti-Mouse CD19-PE was used as the secondary antibody.