Anti-Ly6g 抗体 [RB6-8C5] (Cy5 ®) (ab24985)

製品の概要

  • 製品名
    Anti-Ly6g antibody [RB6-8C5] (Cy5 ®)
    Ly6g 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [RB6-8C5] to Ly6g (Cy5 ®)
  • 標識
    Cy5 ®. Ex: 650nm, Em: 667nm
  • アプリケーション
    適用あり: Flow Cyt, IHC-Fr, IP, WBmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 特記事項
    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab24985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt
IHC-Fr
IP
WB
  • 追加情報
    Flow Cyt: Use 0.2µg for 106 cells.
    IHC-Fr: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 21 kDa.
    Complement-dependent cytotoxicity assays: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性
      Ly6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells.
    • 細胞内局在
      Attached to the membrane by a GPI-anchor.
    • 参照データベース
    • 別名
      • Gr-1 antibody
      • Gr1 antibody
      • Ly-6G.1 antibody
      • Ly6G antibody
      • Lymphocyte antigen 6 complex locus G antibody
      • Lymphocyte antigen 6G antibody
      see all

    Anti-Ly6g antibody [RB6-8C5] (Cy5 ®) (ab24985) 使用論文

    ab24985 has not yet been referenced specifically in any publications.

    Product Wall

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate
    Sample
    Rat Tissue sections (Brain)
    Specification
    Brain
    Permeabilization
    Yes - 0.4% Triton X-100
    Fixative
    Formaldehyde
    Username

    Dr. S Gajavelli

    Verified customer

    投稿 Aug 20 2014

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate
    Sample
    Mouse Tissue sections (lung)
    Specification
    lung
    Permeabilization
    Yes - Triton X-100
    Fixative
    Formalin/PFA-fixed paraffin-embedded sections
    Username

    Abcam user community

    Verified customer

    投稿 May 02 2014

    Thank you very much for your interest in Abcam and our products..

    To our knowledge,the following antibodies you listed have not been tested in IHC-P. Tested applications and species are listed on the individual datasheets. These are updated ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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