The details of the immunogen for this antibody are not available.
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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Depletion: Use at an assay dependent dilution.
Flow Cyt: Use <0.2µg for 106 cells.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 14 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Ly6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells.