Anti-Ly6g 抗体 [RB6-8C5] (Allophycocyanin) (ab25273)

製品の概要

  • 製品名Anti-Ly6g antibody [RB6-8C5] (Allophycocyanin)
    Ly6g 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [RB6-8C5] to Ly6g (Allophycocyanin)
  • 標識Allophycocyanin. Ex: 645nm, Em: 660nm
  • アプリケーション適用あり: Flow Cyt, IHC-Fr, IP, WBmore details
  • 種交差性
    交差種: Mouse, Human, Cynomolgus Monkey
  • 免疫原

    The details of the immunogen for this antibody are not available.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab25273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt
IHC-Fr
IP
WB
  • 追加情報Flow Cyt: Use at an assay dependent dilution. Use up to 0.2ug/106 cells.
    IHC-Fr: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 21-25 kDa.
    In vitro complement-dependent cytotoxicity assays: Use at an assay dependent dilution.
    In vivo depletion of Ly-6G positive cells: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性Ly6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells.
    • 細胞内局在Attached to the membrane by a GPI-anchor.
    • 参照データベース
    • 別名
      • Gr-1 antibody
      • Gr1 antibody
      • Ly-6G.1 antibody
      • Ly6G antibody
      • Lymphocyte antigen 6 complex locus G antibody
      • Lymphocyte antigen 6G antibody
      see all

    Anti-Ly6g antibody [RB6-8C5] (Allophycocyanin) 画像

    • Flow Cytometry analysis of BALB/c mouse bone marrow cells labeling Mouse Ly-6G with ab25273 at 0.01 μg/106 cells. A Rat Anti-Mouse CD19-FITC was used as the secondary antibody.

    • ab25273 at a 1/100 dilution detecting Ly6g in human buffy coat cells by Flow Cytometry.

      See Abreview

    • ab25273 at a 1/100 dilution detecting Ly6g (Gr1) in murine blood cells by Flow Cytometry.

      See Abreview

    • ab25273 staining Ly6g in Mouse spleen tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 5% Goat serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 5% Goat serum) for 1 hour at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rat IgG polyclonal (1/400) was used as the secondary antibody.

      See Abreview

    Anti-Ly6g antibody [RB6-8C5] (Allophycocyanin) (ab25273) 使用論文

    ab25273 has not yet been referenced specifically in any publications.

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    Thank you for submitting an Abreview for ab25273. As you may have noticed, your review has now been published on our website.
    Since you obtained poor results using the antibody in an untested species (dog), we would like to follow up on this to see...

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    Abcam has not validated the combination of species/application used in this Abreview.
    Application Flow Cytometry
    Sample Dog Cell (from whole blood)
    Specification from whole blood
    Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
    Sample buffer: PBS/BSA 1%
    Fixation Paraformaldehyde
    Permeabilization No
    Gating Strategy alive cells
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    Abcam user community

    Verified customer

    投稿 Nov 06 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Sample Cynomolgus Monkey Cell (cells from whole blood)
    Specification cells from whole blood
    Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
    Sample buffer: PBS/BSA 1%
    Fixation Paraformaldehyde
    Permeabilization No
    Gating Strategy alive neutrophils
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    Abcam user community

    Verified customer

    投稿 Nov 02 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Spleen from immune mouse)
    Specification Spleen from immune mouse
    Fixative Acetone
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    Verified customer

    投稿 Apr 16 2012

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Flow Cytometry
    Sample Rat Cell (Splenocytes)
    Specification Splenocytes
    Preparation Cell harvesting/tissue preparation method: spleen was mashed
    Sample buffer: PBS/BSA 1%/Azide 0.01%
    Permeabilization No
    Gating Strategy alive macrophages
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    Abcam user community

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    投稿 Apr 16 2012

    Thank you for contacting Abcam. I am sorry to hear you have experienced difficulty obtaining satisfactory results from this antibody.

    I would be happy to arrange a Free of Charge Replacement of this product for you. We do have one product ...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Sample Mouse Cell (cells from mouse blood)
    Specification cells from mouse blood
    Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
    Sample buffer: PBS/BSA 1%
    Permeabilization No
    Gating Strategy on neutrophils
    Username

    Abcam user community

    Verified customer

    投稿 Dec 20 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Sample Human Cell (cell from buffy coat)
    Specification cell from buffy coat
    Preparation Cell harvesting/tissue preparation method: none
    Sample buffer: PBS/BSA 1%
    Permeabilization No
    Gating Strategy on granulocytes
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    Verified customer

    投稿 Dec 20 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"