Anti-Ly6g 抗体 [RB6-8C5] (Allophycocyanin) (ab25273)

製品の概要

  • 製品名
    Anti-Ly6g antibody [RB6-8C5] (Allophycocyanin)
    Ly6g 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [RB6-8C5] to Ly6g (Allophycocyanin)
  • 標識
    Allophycocyanin. Ex: 645nm, Em: 660nm
  • アプリケーション
    適用あり: Flow Cytmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab25273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration.

ターゲット情報

  • 関連性
    Ly6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells.
  • 細胞内局在
    Attached to the membrane by a GPI-anchor.
  • 参照データベース
  • 別名
    • Gr-1 antibody
    • Gr1 antibody
    • Ly-6G.1 antibody
    • Ly6G antibody
    • Lymphocyte antigen 6 complex locus G antibody
    • Lymphocyte antigen 6G antibody
    see all

プロトコール

参考文献

ab25273 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for your message and for providing this further information.

In order to arrange this, I would appreciate if you could confirm your order number and date of purchase?

Thank you for your continued cooperation. I look ...

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Thank you for submitting an Abreview for ab25273. As you may have noticed, your review has now been published on our website.
Since you obtained poor results using the antibody in an untested species (dog), we would like to follow up on this to see...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Dog Cell (from whole blood)
Specification
from whole blood
Preparation
Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
alive cells
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Abcam user community

Verified customer

投稿 Nov 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Cynomolgus Monkey Cell (cells from whole blood)
Specification
cells from whole blood
Preparation
Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
alive neutrophils
Username

Abcam user community

Verified customer

投稿 Nov 02 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Spleen from immune mouse)
Specification
Spleen from immune mouse
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Apr 16 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Rat Cell (Splenocytes)
Specification
Splenocytes
Preparation
Cell harvesting/tissue preparation method: spleen was mashed
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization
No
Gating Strategy
alive macrophages
Username

Abcam user community

Verified customer

投稿 Apr 16 2012

Thank you for contacting Abcam. I am sorry to hear you have experienced difficulty obtaining satisfactory results from this antibody.

I would be happy to arrange a Free of Charge Replacement of this product for you. We do have one product ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Mouse Cell (cells from mouse blood)
Specification
cells from mouse blood
Preparation
Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Permeabilization
No
Gating Strategy
on neutrophils
Username

Abcam user community

Verified customer

投稿 Dec 20 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (cell from buffy coat)
Specification
cell from buffy coat
Preparation
Cell harvesting/tissue preparation method: none
Sample buffer: PBS/BSA 1%
Permeabilization
No
Gating Strategy
on granulocytes
Username

Abcam user community

Verified customer

投稿 Dec 20 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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