製品の概要

  • 製品名Anti-Ly6g antibody [RB6-8C5]
    Ly6g 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [RB6-8C5] to Ly6g
  • アプリケーション適用あり: IHC-Fr, IP, , Functional Studies, IHC-P, Flow Cytmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Tissue/ cell preparation of Normal murine bone marrow (Mouse)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab25377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Depletion Use at an assay dependent concentration.

In vivo depletion of Ly-6G+ cells.

Functional Studies Use at an assay dependent concentration.

In vitro complement-dependent cytotoxicity assays.

IHC-P Use at an assay dependent concentration. PubMed: 25126943
Flow Cyt Use at an assay dependent concentration.

ターゲット情報

  • 関連性Ly6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells.
  • 細胞内局在Attached to the membrane by a GPI-anchor.
  • 参照データベース
  • 別名
    • Gr-1 antibody
    • Gr1 antibody
    • Ly-6G.1 antibody
    • Ly6G antibody
    • Lymphocyte antigen 6 complex locus G antibody
    • Lymphocyte antigen 6G antibody
    see all

Anti-Ly6g antibody [RB6-8C5] 画像

  • ab25377 staining Ly6g in Mouse placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% BSA for 30 minutes at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100 in 1% BSA in PBS) for 12 hours at 4°C. ab6734,  a HRP-conjugated Rabbit anti-rat IgG polyclonal (1/200) was used as the secondary antibody.

     

    See Abreview

  • Immunohistochemistry (Frozen sections) analysis of Mouse frozen spleen tissue labeling Ly6g with ab25377. Right: Rat Anti-Mouse Ly-6G-UNLB followed by Mouse Anti-Rat IgG2b-FITC. Left: Isotype Control, Rat IgG2b-UNLB followed by Mouse Anti-Rat IgG2b-FITC. Blue - DAPI.

  • ab25377 staining Ly6C in Mouse heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 0.5% BSA; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100) for 1 hour at room temperature. A Peroxidase-conjugated anti-rat Ig polyclonal was used as the secondary antibody.

  • ab25377 staining Ly6g in Mouse bone tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with PBS, BSA 1% + NGS5% for 30 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in PBS + BSA 1%) for 9 hours at 4°C. A Biotin-conjugated Goat anti-rat polyclonal (1/500) was used as the secondary antibody.

    See Abreview

Anti-Ly6g antibody [RB6-8C5] (ab25377) 使用論文

This product has been referenced in:
  • Gonzalez JM  et al. Statins prevent cervical remodeling, myometrial contractions and preterm labor through a mechanism that involves hemoxygenase-1 and complement inhibition. Mol Hum Reprod 20:579-89 (2014). Mouse . Read more (PubMed: 24623738) »
  • Ohm IK  et al. Toll-like receptor 9-activation during onset of myocardial ischemia does not influence infarct extension. PLoS One 9:e104407 (2014). IHC-P ; Mouse . Read more (PubMed: 25126943) »

See all 18 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step PBS BSA 1% NGS 5% as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Sample Mouse Tissue sections (bone)
Specification bone
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Mar 24 2014

We state to perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol as done in PubMed: 21713436. This publication used a citrate buffer pH6 so we would recommend you try this buffer also.
...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step Heat mediated
Sample Mouse Tissue sections (placenta)
Specification placenta
Permeabilization No
Fixative Formaldehyde
Username

Dr. Varkha Agrawal

Verified customer

投稿 Nov 11 2013

Thank you for your inquiry.

I am happy to confirm that ab25377 is tested and guaranteed for mouse samples and IHC-P. WE do not know of a special protocol for distal colon from mouse and have not tested this specifically. Maybe the literature ...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results with the Anti-Ly6g antibody [RB6-8C5] (ab25377).

I would like to reassure you that this antibody is tested and covered by ou...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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I have performed a BLAST search with the Ly6g sequence, and it looks like the sequence of Ly6g and Ly6c are 60 to 64% identical, it is rather unlikely that this antibody will react withLy6c:We recommend that alignment should be over 85% to predict that...

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Thank you for contacting us.

Yes, has been used successfully on mouse, and as well in IHC-P. therefore, you are covered by our Abpromise.

We haven't tested IHC-P ourselves, but the following reference used the antibody in IHC-P under ...

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Thank you for contacting us.

For ab25377, I would recommend a starting WB concentration of 1ug/mL. The final concentration will need to be optimized to your specific protocol and samples.

This clone has been used in a number of refe...

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Thank you for your call yesterday and for your patience while I have looked into your enquiry.

I have found a couple of potential markers: beta glucan receptor and neutrophil elastase. Beta glucan receptor is expressed by cells of the monocyt...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"