Anti-Ly6c 抗体 [ER-MP 20] (FITC) (ab15686)

製品の概要

  • 製品名Anti-Ly6c antibody [ER-MP 20] (FITC)
    Ly6c 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [ER-MP 20] to Ly6c (FITC)
  • 標識FITC. Ex: 493nm, Em: 528nm
  • 特異性ab15686 recognizes murine Ly6C, a 14kD differentiation antigen, which is expressed on macrophage/dendritic cell precursors in midstage development (late CFUM, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B and T lymphocytes. It is ideally suited for the detection of monocytes in bone marrow samples by FACS. It also identifies activated macrophages in inflammatory tissues where the simultaneous use of the murine pan-macrophage marker is recommended.
  • アプリケーション適用あり: ICC/IF, Flow Cyt, ICCmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Tissue, cells or virus corresponding to Mouse Ly6c. Also; SwissProt ID P0CW03.
    Database link: P0CW02

  • エピトープSpleen cells from immunised rats were fused with cells of the Y3Ag1.2.3 myeloma cell line.
  • 特記事項

    ab15686 is useful for the detection of macrophage precursor cells in the mid-stage development stage (late CFU-M, monoblasts and monocytes). It is highly suitable for the detection of monocytes in bone marrow samples by FACS.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab15686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
Flow Cyt 1/10 - 1/20. ab18446-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC 1/200.

ターゲット情報

  • 関連性Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Mice with the Ly6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly6C+ and CD8+Ly6C+ cells, while Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly6C+ lymphocytes.
  • 細胞内局在Cell membrane; Lipid-anchor, GPI-anchor.
  • 参照データベース
  • 別名
    • Ly 6c antibody
    • Ly6c protein antibody
    • Ly6c1 antibody
    • Ly6c2 antibody
    • Lymphocyte antigen 6 complex antibody
    • Lymphocyte antigen 6 complex locus C antibody
    • Lymphocyte antigen 6C antibody
    • Lymphocyte antigen 6C1 antibody
    • Lymphocyte antigen 6C2 antibody
    • Lymphocyte antigen Ly 6C antibody
    see all

Anti-Ly6c antibody [ER-MP 20] (FITC) (ab15686) 使用論文

ab15686 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (MEF)
Specification MEF
Fixative none, used on live cells
Permeabilization No
Blocking step Serum as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
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投稿 Oct 03 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"