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Our Abpromise guarantee covers the use of ab24361 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 60, 80 kDa (predicted molecular weight: 51 kDa).|
|IHC-P||Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
ab24361 at 3µg/ml staining LXR beta in human spleen tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). Tissue underwent antigen retrieval in steam in citrate buffer pH 6.0. AP staining procedure was used for detection.