Anti-LXR alpha 抗体 [PPZ0412] - ChIP Grade (ab41902)

製品の概要

  • 製品名Anti-LXR alpha antibody [PPZ0412] - ChIP Grade
    LXR alpha 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [PPZ0412] to LXR alpha - ChIP Grade
  • 特異性This antibody does not recognize human LXR beta.
  • アプリケーション適用あり: ChIP, ICC/IF, WB, ELISA, IP, IHC-P, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    E.coli-expressed recombinant fragment, corresponding to amino acids 164-447 of Human LXR alpha.

  • ポジティブ・コントロール
    • Rat hepatocyte and kupffer cell. Rat spleen macrophage.

アプリケーション

Our Abpromise guarantee covers the use of ab41902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ChIP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
EMSA Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa.
ELISA Use a concentration of 0.2 µg/ml.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 20 - 40 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Orphan receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8.
  • 組織特異性Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals.
  • 配列類似性Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Liver X receptor alpha antibody
    • LXR a antibody
    • LXRA antibody
    • NR1H3 antibody
    • NR1H3_HUMAN antibody
    • Nuclear receptor subfamily 1 group H member 3 antibody
    • Oxysterols receptor LXR alpha antibody
    • Oxysterols receptor LXR-alpha antibody
    • RLD 1 antibody
    • RLD1 antibody
    see all

Anti-LXR alpha antibody [PPZ0412] - ChIP Grade 画像

  • ab41902 staining LXR alpha in Rat Hepatocyte and Kupffer cell paraffin section (20-40ug/mL) by Immunohistochemistry, formalin-fixed paraffin embedded sections.
  • ab41902 staining LXR alpha in rat spleen macrophage cell paraffin section (20-40ug/mL) by Immunohistochemistry, formalin-fixed paraffin embedded sections.
  • All lanes : Anti-LXR alpha antibody [PPZ0412] - ChIP Grade (ab41902) at 1/1000 dilution (in 2.5% BSA + TBS for 16 hours at 4°C)

    Lane 1 : RAW 264.7 murine macrophage cell line lysate without endogenous LXRalpha
    Lane 2 : RAW 264.7 murine macrophage cell line lysate overexpressing human FLAG-LXRalpha

    Lysates/proteins at 35 µg per lane.

    Secondary
    An HRP-conjugated Goat anti-mouse IgG polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 50 kDa


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Overlay histogram showing HepG2 cells stained with ab41902 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab41902, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-LXR alpha antibody [PPZ0412] - ChIP Grade (ab41902) 使用論文

This product has been referenced in:
  • Bouttier M  et al. Alu repeats as transcriptional regulatory platforms in macrophage responses to M. tuberculosis infection. Nucleic Acids Res N/A:N/A (2016). Human . Read more (PubMed: 27604870) »
  • Vinod M  et al. miR-206 controls LXRa expression and promotes LXR-mediated cholesterol efflux in macrophages. Biochim Biophys Acta 1841:827-35 (2014). WB ; Mouse, Human . Read more (PubMed: 24603323) »

See all 14 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (monocyte-derived macrophage)
Gel Running Conditions Non-reduced Denaturing
Loading amount 100000 cells
Specification monocyte-derived macrophage
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jun 29 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HepG2 cells)
Loading amount 30 µg
Specification HepG2 cells
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 8% · Temperature: 20°C
Username

Mr. Dongil Kim

Verified customer

投稿 Apr 08 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (lung)
Loading amount 30 µg
Specification lung
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Ms. JIHUN LEE

Verified customer

投稿 Jan 10 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Mono-mac 6)
Loading amount 50 µg
Specification Mono-mac 6
Treatment TO-901317
Gel Running Conditions Reduced Denaturing (8%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Dr. Elizabeth Kilby

Verified customer

投稿 Dec 19 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (THP1 monocyte-derived macrophages)
Specification THP1 monocyte-derived macrophages
Fixative Paraformaldehyde
Permeabilization Yes - 0.25% Triton X-100 in PBS
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Dr. Elizabeth Kilby

Verified customer

投稿 Oct 17 2012

As requested, one unit of ab106464 has been added to the next order to be shipped to our distributor.
I wish all the best with your reseach work.

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.
The details provided will enable us to investigate this case and will provide us with vital infor...

Read More

Thank you for contacting us.

I have been in contact with the lab on your behalf requesting the blocking peptide for ab41902. Unfortunately this is not available. I am very sorry for any inconvenience and will continue to work with you to g...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Rat liver tissue)
Loading amount 120 µg
Specification Rat liver tissue
Treatment ovariectomy
Gel Running Conditions Reduced Denaturing (10%)
Blocking step 5% milk in TBS-T(0.1%) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mrs. Smitha Koshy

Verified customer

投稿 May 21 2012

In der folgenden Publikation wurde der Klon validiert:


Expression of LXR alpha Protein in Human Atherosclerotic Lesions

Watanabe Y, et al.

Arterioscler Thromb Vasc Biol. 2005; 25(3):622-627

1-10 of 28 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"