Anti-LXR alpha 抗体 [PPZ0412] - ChIP Grade (ab41902)

製品の概要

  • 製品名
    Anti-LXR alpha antibody [PPZ0412] - ChIP Grade
    LXR alpha 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [PPZ0412] to LXR alpha - ChIP Grade
  • 特異性
    This antibody does not recognize human LXR beta.
  • アプリケーション
    適用あり: ChIP, ICC/IF, WB, ELISA, IP, IHC-P, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    E.coli-expressed recombinant fragment, corresponding to amino acids 164-447 of Human LXR alpha.

  • ポジティブ・コントロール
    • Rat hepatocyte and kupffer cell. Rat spleen macrophage.

アプリケーション

Our Abpromise guarantee covers the use of ab41902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ChIP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
EMSA Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa.
ELISA Use a concentration of 0.2 µg/ml.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 20 - 40 µg/ml.
Flow Cyt Use 1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    Orphan receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8.
  • 組織特異性
    Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals.
  • 配列類似性
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Liver X receptor alpha antibody
    • LXR a antibody
    • LXRA antibody
    • NR1H3 antibody
    • NR1H3_HUMAN antibody
    • Nuclear receptor subfamily 1 group H member 3 antibody
    • Oxysterols receptor LXR alpha antibody
    • Oxysterols receptor LXR-alpha antibody
    • RLD 1 antibody
    • RLD1 antibody
    see all

画像

  • Cross-linking (X-ChIP) analysis of RAW 264.7 murine macrophage cell line nuclear cell lysate labeling LXR alpha with ab41902. 10 minutes duration of cross-linking using formaldehyde as cross-linking agent. Postive control: DMSO treated cells and T091317 treated cells (RAW overexpressing human LXRalpha); nehative control: DMSO treated cells and T091317 treated cells (RAW without overexpression of human LXRalpha, RAW VO). Samples were immunoprecipitated with mouse IgG.

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  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue sections labeling LXR alpha with ab41902 at 1/100 dilution. Tissue was fixed with formaldehyde and permeabilized with Tween 20. Heat mediated antigen retrieval was performed using TRIS-EDTA Buffer. Tissue sections were incubated with Anti-LXR alpha antibody [PPZ0412] - ChIP Grade (ab41902) for 30 minutes at 20°C in diluent from DAKO. An undiluted HRP conjugated horse anti-mouse secondary antibody was used.

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  • All lanes : Anti-LXR alpha antibody [PPZ0412] - ChIP Grade (ab41902) at 1/1000 dilution

    Lane 1 : Bone marrow-derivate of macrophages from C57Bl6 mice treated with DMSO
    Lane 2 : Bone marrow-derivate of macrophages from C57Bl6 mice treated with T091317

    Lysates/proteins at 40 µg per lane.

    Secondary
    Goant anti-murine IgG HRP (Undiluted)
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa


    Exposure time : 5 minutes

    This image is courtesy of an anonymous abreview.

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  • ab41902 staining LXR alpha in rat spleen macrophage cell paraffin section (20-40ug/mL) by Immunohistochemistry, formalin-fixed paraffin embedded sections.
  • Immunocytochemistry/ Immunofluorescence analysis of human THP1 monocyte-derived macrophages labeling LXR alpha with ab41902 at 1/50. Cells were fixed with paraformaldehyde and permeabilized with 0.25% Triton X-100 in PBS. Next the cells were blocked with 10% BSA for 30 minutes at room temperature, followed by incubation with primary antibody in 10% BSA/0.05% Triton/0.3M glycine in PBS for 16 hours at 4°C. A polyclonal donkey anti-mouse Alexa Fluor® 555 secondary antibody was used at 1/300 dilution. DAPI nuclear counter stain.

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  • ab41902 staining LXR alpha in Rat Hepatocyte and Kupffer cell paraffin section (20-40ug/mL) by Immunohistochemistry, formalin-fixed paraffin embedded sections.
  • All lanes : Anti-LXR alpha antibody [PPZ0412] - ChIP Grade (ab41902) at 1/1000 dilution (in 2.5% BSA + TBS for 16 hours at 4°C)

    Lane 1 : RAW 264.7 murine macrophage cell line lysate without endogenous LXRalpha
    Lane 2 : RAW 264.7 murine macrophage cell line lysate overexpressing human FLAG-LXRalpha

    Lysates/proteins at 35 µg per lane.

    Secondary
    An HRP-conjugated Goat anti-mouse IgG polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 50 kDa


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    Blocking step: 5% Milk for 1 hour at 25°C

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  • Overlay histogram showing HepG2 cells stained with ab41902 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab41902, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

参考文献

This product has been referenced in:
  • Song L  et al. Phytosterol esters attenuate hepatic steatosis in rats with non-alcoholic fatty liver disease rats fed a high-fat diet. Sci Rep 7:41604 (2017). WB ; Rat . Read more (PubMed: 28169366) »
  • Ikhlef S  et al. Human paraoxonase 1 overexpression in mice stimulates HDL cholesterol efflux and reverse cholesterol transport. PLoS One 12:e0173385 (2017). WB ; Mouse . Read more (PubMed: 28278274) »

See all 29 Publications for this product

レビューと Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (monocyte-derived macrophage)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
100000 cells
Specification
monocyte-derived macrophage
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jun 29 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2 cells)
Loading amount
30 µg
Specification
HepG2 cells
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 8% · Temperature: 20°C
Username

Mr. Dongil Kim

Verified customer

投稿 Apr 08 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (lung)
Loading amount
30 µg
Specification
lung
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Ms. Jihun Lee

Verified customer

投稿 Jan 10 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Mono-mac 6)
Loading amount
50 µg
Specification
Mono-mac 6
Treatment
TO-901317
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Dr. Elizabeth Kilby

Verified customer

投稿 Dec 19 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (THP1 monocyte-derived macrophages)
Specification
THP1 monocyte-derived macrophages
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.25% Triton X-100 in PBS
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Dr. Elizabeth Kilby

Verified customer

投稿 Oct 17 2012

As requested, one unit of ab106464 has been added to the next order to be shipped to our distributor.
I wish all the best with your reseach work.

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.
The details provided will enable us to investigate this case and will provide us with vital infor...

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Thank you for contacting us.

I have been in contact with the lab on your behalf requesting the blocking peptide for ab41902. Unfortunately this is not available. I am very sorry for any inconvenience and will continue to work with you to g...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Rat liver tissue)
Loading amount
120 µg
Specification
Rat liver tissue
Treatment
ovariectomy
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
5% milk in TBS-T(0.1%) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mrs. Smitha Koshy

Verified customer

投稿 May 21 2012

In der folgenden Publikation wurde der Klon validiert:


Expression of LXR alpha Protein in Human Atherosclerotic Lesions

Watanabe Y, et al.

Arterioscler Thromb Vasc Biol. 2005; 25(3):622-627

1-10 of 28 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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