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E.coli-expressed recombinant fragment, corresponding to amino acids 164-447 of Human LXR alpha.
Our Abpromise guarantee covers the use of ab41902 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|EMSA||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa.|
|ELISA||Use a concentration of 0.2 µg/ml.|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 20 - 40 µg/ml.|
|Flow Cyt||Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
Cross-linking (X-ChIP) analysis of RAW 264.7 murine macrophage cell line nuclear cell lysate labeling LXR alpha with ab41902. 10 minutes duration of cross-linking using formaldehyde as cross-linking agent. Postive control: DMSO treated cells and T091317 treated cells (RAW overexpressing human LXRalpha); nehative control: DMSO treated cells and T091317 treated cells (RAW without overexpression of human LXRalpha, RAW VO). Samples were immunoprecipitated with mouse IgG.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue sections labeling LXR alpha with ab41902 at 1/100 dilution. Tissue was fixed with formaldehyde and permeabilized with Tween 20. Heat mediated antigen retrieval was performed using TRIS-EDTA Buffer. Tissue sections were incubated with Anti-LXR alpha antibody [PPZ0412] - ChIP Grade (ab41902) for 30 minutes at 20°C in diluent from DAKO. An undiluted HRP conjugated horse anti-mouse secondary antibody was used.
This image is courtesy of an anonymous abreview.
Immunocytochemistry/ Immunofluorescence analysis of human THP1 monocyte-derived macrophages labeling LXR alpha with ab41902 at 1/50. Cells were fixed with paraformaldehyde and permeabilized with 0.25% Triton X-100 in PBS. Next the cells were blocked with 10% BSA for 30 minutes at room temperature, followed by incubation with primary antibody in 10% BSA/0.05% Triton/0.3M glycine in PBS for 16 hours at 4°C. A polyclonal donkey anti-mouse Alexa Fluor® 555 secondary antibody was used at 1/300 dilution. DAPI nuclear counter stain.
This image is courtesy of an anonymous AbreviewBlocking step: 5% Milk for 1 hour at 25°C