Anti-LXR alpha 抗体 [PPZ0412] (ab41902)
Key features and details
- Mouse monoclonal [PPZ0412] to LXR alpha
- Suitable for: ICC/IF, ELISA, IP, ChIP, GSA, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-LXR alpha antibody [PPZ0412]
LXR alpha 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [PPZ0412] to LXR alpha -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, ELISA, IP, ChIP, GSA, IHC-P, Flow Cytmore details
適用なし: WB -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Human LXR alpha aa 164-447.
Database link: Q13133 -
ポジティブ・コントロール
- Rat hepatocyte and kupffer cell. Rat spleen macrophage.
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特記事項
This product was changed from ascites to tissue culture supernatant on 3rd April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Physiological saline. -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
PPZ0412 -
アイソタイプ
IgG2a -
研究分野
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Orphan Nuclear Receptors
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab41902の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (1) |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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|
IP |
Use at an assay dependent concentration.
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ChIP | (1) |
Use at an assay dependent concentration.
|
GSA |
Use at an assay dependent concentration.
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EMSA |
Use at an assay dependent concentration.
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|
IHC-P | (2) |
Use at an assay dependent concentration.
|
Flow Cyt |
Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
特記事項 |
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ICC/IF
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
ChIP
Use at an assay dependent concentration. |
GSA
Use at an assay dependent concentration. |
EMSA
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Orphan receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. -
組織特異性
Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals. -
配列類似性
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 10062 Human
- Entrez Gene: 22259 Mouse
- Entrez Gene: 58852 Rat
- Omim: 602423 Human
- SwissProt: Q13133 Human
- SwissProt: Q9Z0Y9 Mouse
- SwissProt: Q62685 Rat
- Unigene: 438863 Human
see all -
別名
- Liver X receptor alpha antibody
- LXR a antibody
- LXRA antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue sections labeling LXR alpha with ab41902 at 1/100 dilution. Tissue was fixed with formaldehyde and permeabilized with Tween 20. Heat mediated antigen retrieval was performed using TRIS-EDTA Buffer. Tissue sections were incubated with Anti-LXR alpha antibody [PPZ0412] - ChIP Grade (ab41902) for 30 minutes at 20°C in diluent from DAKO. An undiluted HRP conjugated horse anti-mouse secondary antibody was used.
This image was generated using the ascites version of the product.
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ab41902 staining LXR alpha in rat spleen macrophage cell paraffin section (20-40ug/mL) by Immunohistochemistry, formalin-fixed paraffin embedded sections.
This image was generated using the ascites version of the product.
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ab41902 staining LXR alpha in Rat Hepatocyte and Kupffer cell paraffin section (20-40ug/mL) by Immunohistochemistry, formalin-fixed paraffin embedded sections.
This image was generated using the ascites version of the product.
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Overlay histogram showing HepG2 cells stained with ab41902 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab41902, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (78)
ab41902 は 78 報の論文で使用されています。
- Chinetti G et al. Diabetes-Induced Changes in Macrophage Biology Might Lead to Reduced Risk for Abdominal Aortic Aneurysm Development. Metabolites 12:N/A (2022). PubMed: 35208203
- Lin CY et al. The Citrus Flavonoid Nobiletin Downregulates Angiopoietin-like Protein 3 (ANGPTL3) Expression and Exhibits Lipid-Modulating Effects in Hepatic Cells and Adult Zebrafish Models. Int J Mol Sci 23:N/A (2022). PubMed: 36293338
- Gao WY et al. Tanshinone IIA Downregulates Lipogenic Gene Expression and Attenuates Lipid Accumulation through the Modulation of LXRa/SREBP1 Pathway in HepG2 Cells. Biomedicines 9:N/A (2021). PubMed: 33806955
- Pu Y et al. MicroRNA-325 facilitates atherosclerosis progression by mediating the SREBF1/LXR axis via KDM1A. Life Sci 277:119464 (2021). PubMed: 33811891
- Zhang X et al. Tumor Suppressor Gene XEDAR Promotes Differentiation and Suppresses Proliferation and Migration of Gastric Cancer Cells Through Upregulating the RELA/LXRα Axis and Deactivating the Wnt/β-Catenin Pathway. Cell Transplant 30:963689721996346 (2021). PubMed: 33637015