製品の概要

アプリケーション

Our Abpromise guarantee covers the use of ab28478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Predicted molecular weight: 50 kDa.

ターゲット情報

  • 機能Orphan receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8.
  • 組織特異性Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals.
  • 配列類似性Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Liver X receptor alpha antibody
    • LXR a antibody
    • LXRA antibody
    • NR1H3 antibody
    • NR1H3_HUMAN antibody
    • Nuclear receptor subfamily 1 group H member 3 antibody
    • Oxysterols receptor LXR alpha antibody
    • Oxysterols receptor LXR-alpha antibody
    • RLD 1 antibody
    • RLD1 antibody
    see all

Anti-LXR alpha antibody 画像

  • Anti-LXR alpha antibody (ab28478) (at a dilution of 1/500) + mouse liver samples

    Predicted band size : 50 kDa
    Observed band size : 50 kDa

Anti-LXR alpha antibody (ab28478) 使用論文

This product has been referenced in:
  • Pirinen E  et al. Activated polyamine catabolism leads to low cholesterol levels by enhancing bile acid synthesis. Amino Acids 38:549-60 (2010). WB ; Mouse . Read more (PubMed: 19956992) »
  • Martin IV  et al. Bile acid retention and activation of endogenous hepatic farnesoid-X-receptor in the pathogenesis of fatty liver disease in ob/ob-mice. Biol Chem 391:1441-9 (2010). WB ; Mouse . Read more (PubMed: 20868235) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HepG2, 293T, MEF)
Loading amount 20 µg
Specification HepG2, 293T, MEF
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7% · Temperature: 20°C
Username

Mr. Dongil Kim

Verified customer

投稿 Feb 01 2013

Thank you for taking the time to contact us. I am sorry to hear the customer has some concerns regarding the results from this antibody.

In answer to your question, the alignment of the immunogen of ab28478 with beta subunit is 26%. So it is...

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I'm sorry to hear you have been experiencing problems with ab28478 in western blotting. Thank you for providing your protocol details which enabled me to understand rapidly how you performed your experiment. I have a few suggestions detailed below ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"