Anti-Lumican 抗体 [EPR8898(2)] (ab168348)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8898(2)] to Lumican
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Lumican antibody [EPR8898(2)]
Lumican 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR8898(2)] to Lumican -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
適用なし: IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide within Human Lumican aa 250-350. The exact sequence is proprietary.
Database link: P51884 -
ポジティブ・コントロール
- WB: Saos-2, fetal skin, fetal heart, Jurkat, Y79 and HEK-293T lysates. IHC-P: Human kidney and Human skin tissues. ICC/IF: Saos-2 cells. Flow Cyt (intra): HEK-293T cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR8898(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab168348の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 38 kDa).
|
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IHC-P | (6) |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
ICC/IF | (3) |
1/250 - 1/500.
|
特記事項 |
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 38 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/250 - 1/500. |
ターゲット情報
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組織特異性
Cornea and other tissues. -
配列類似性
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.
Contains 11 LRR (leucine-rich) repeats.
Contains 1 LRRNT domain. -
発生段階
Present in the extracellular matrix of human articular cartilage at all ages, although its abundance is far greater in the adult. In the adult cartilage lumican exists predominantly in a glycoprotein form lacking keratan sulfate, whereas the juvenile form of the molecule is a proteoglycan. -
翻訳後修飾
Sulfated on tyrosine residue(s). -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 4060 Human
- Entrez Gene: 17022 Mouse
- Entrez Gene: 81682 Rat
- Omim: 600616 Human
- SwissProt: P51884 Human
- SwissProt: P51885 Mouse
- SwissProt: P51886 Rat
- Unigene: 406475 Human
see all -
別名
- Keratan sulfate proteoglycan lumican antibody
- KSPG lumican antibody
- LDC antibody
see all
画像
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All lanes : Anti-Lumican antibody [EPR8898(2)] (ab168348) at 1/1000 dilution
Lane 1 : Saos-2 lysate
Lane 2 : Fetal skin lysate
Lane 3 : Fetal heart lysate
Lane 4 : Jurkat lysate
Lane 5 : Y79 lysate
Lane 6 : 293T lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 38 kDa
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemical analysis of paraffin embedded Human skin tissue labeling Lumican with ab168348 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent analysis of Saos-2 cells labeling Lumican with ab168348 at 1/250.
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Intracellular flow cytometrical analysis of permeabilized 293T cells with ab168348 antibody at a dilution of 1/100 (red) or a rabbit IgG (negative) (green).
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Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling Lumican with ab168348 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (30)
ab168348 は 30 報の論文で使用されています。
- Chow SE et al. Nuclear p120 catenin is a component of the perichromosomal layer and coordinates sister chromatid segregation during mitosis in lung cancer cells. Cell Death Dis 13:526 (2022). PubMed: 35660718
- Wang K et al. Lumican is elevated in the lung in human and experimental acute respiratory distress syndrome and promotes early fibrotic responses to lung injury. J Transl Med 20:392 (2022). PubMed: 36059026
- Wu J et al. Effects of lumican expression on the apoptosis of scleral fibroblasts: In vivo and in vitro experiments. Exp Ther Med 21:495 (2021). PubMed: 33791004
- Kafarnik C et al. Canine Corneal Stromal Cells Have Multipotent Mesenchymal Stromal Cell Properties In Vitro. Stem Cells Dev 29:425-439 (2020). PubMed: 31973649
- Denes BJ et al. Core Matrisome Protein Signature During Periodontal Ligament Maturation From Pre-occlusal Eruption to Occlusal Function. Front Physiol 11:174 (2020). PubMed: 32194440