製品の概要

  • 製品名Anti-Lumican antibody
    Lumican 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Lumican
  • アプリケーション適用あり: WB, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rabbit, Horse, Chicken, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human Lumican.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: Liver (Human) Tissue Lysate. This antibody gave a positive signal in the following formaldehyde fixed cell lines: HeLa cells.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab98067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
ELISA Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 組織特異性Cornea and other tissues.
  • 配列類似性Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.
    Contains 11 LRR (leucine-rich) repeats.
    Contains 1 LRRNT domain.
  • 発生段階Present in the extracellular matrix of human articular cartilage at all ages, although its abundance is far greater in the adult. In the adult cartilage lumican exists predominantly in a glycoprotein form lacking keratan sulfate, whereas the juvenile form of the molecule is a proteoglycan.
  • 翻訳後修飾Sulfated on tyrosine residue(s).
  • 細胞内局在Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Keratan sulfate proteoglycan lumican antibody
    • KSPG lumican antibody
    • LDC antibody
    • LUM antibody
    • LUM_HUMAN antibody
    • Lumican antibody
    • Lumican precursor antibody
    • SLRR2D antibody
    see all

Anti-Lumican antibody 画像

  • All lanes : Anti-Lumican antibody (ab98067) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Lung (Human) Tissue Lysate
    Lane 3 : Human colon tissue lysate - total protein (ab30051)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 38 kDa
    Additional bands at : 50 kDa (possible glycosylated form),60 kDa (possible glycosylated form).

    Exposure time : 8 minutes
  • ICC/IF image of ab98067 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab98067 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Lumican antibody (ab98067) 使用論文

ab98067 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry
Sample Mouse Cultured Cells (Oligodendrocyte precursor cell line)
Permeabilization Yes - 0.5%
Specification Oligodendrocyte precursor cell line
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative Paraformaldehyde
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Dr. Sueli Marques

Verified customer

投稿 Jan 12 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"