製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Lumican
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 179-228 (AFKGLKSLEY LDLSFNQIAR LPSGLPVSLL TLYLDNNKIS NIPDEYFKRF) of Human Lumican (NP_002336).

  • ポジティブ・コントロール
    • Human fetal liver tissue lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 組織特異性
    Cornea and other tissues.
  • 配列類似性
    Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.
    Contains 11 LRR (leucine-rich) repeats.
    Contains 1 LRRNT domain.
  • 発生段階
    Present in the extracellular matrix of human articular cartilage at all ages, although its abundance is far greater in the adult. In the adult cartilage lumican exists predominantly in a glycoprotein form lacking keratan sulfate, whereas the juvenile form of the molecule is a proteoglycan.
  • 翻訳後修飾
    Sulfated on tyrosine residue(s).
  • 細胞内局在
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Keratan sulfate proteoglycan lumican antibody
    • KSPG lumican antibody
    • LDC antibody
    • LUM antibody
    • LUM_HUMAN antibody
    • Lumican antibody
    • Lumican precursor antibody
    • SLRR2D antibody
    see all

画像

  • Anti-Lumican antibody (ab108286) at 1 µg/ml + Human fetal heart tissue lysate at 10 µg

    Predicted band size : 38 kDa

プロトコール

参考文献

ab108286 has not yet been referenced specifically in any publications.

レビューと Q&A

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse retina, RPE, and eye cup homogenates)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Specification
Mouse retina, RPE, and eye cup homogenates
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Dec 12 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Retinal pigment epithelium, retina)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
20 µg
Specification
Retinal pigment epithelium, retina
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Dec 12 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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