製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to LTBP1
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: Cow, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1350 - 1450 of Human LTBP1.

    (Peptide available as ab87128.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following tissue lysates: Rat Liver; Mouse Pancreas; Mouse Spleen. ]# This antibody gave a positive result in IHC in the following FFPE tissue: Mouse spleen.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab78294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 187 kDa (predicted molecular weight: 187 kDa).
IHC-P Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    May be involved in the assembly, secretion and targeting of TGFB1 to sites at which it is stored and/or activated. May play critical roles in controlling and directing the activity of TGFB1. May have a structural role in the extra cellular matrix (ECM).
  • 組織特異性
    Isoform Long is found in fibroblasts.
  • 配列類似性
    Belongs to the LTBP family.
    Contains 18 EGF-like domains.
    Contains 4 TB (TGF-beta binding) domains.
  • ドメイン
    Associates covalently with small latent TGF-beta complex via domain TB 3.
  • 翻訳後修飾
    Contains hydroxylated asparagine residues.
    Isoform Short N-terminus is blocked.
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 9430031G15Rik antibody
    • 9830146M04 antibody
    • Latent transforming growth factor beta binding protein 1 antibody
    • Latent-transforming growth factor beta-binding protein 1 antibody
    • Latent-transforming growth factor beta-binding protein, isoform 1L antibody
    • Latent-transforming growth factor beta-binding protein, isoform 1S antibody
    • LBT1S antibody
    • LTBP-1 antibody
    • Ltbp1 antibody
    • LTBP1_HUMAN antibody
    • MGC163161 antibody
    • TGF-beta1-BP-1 antibody
    • Tgfb antibody
    • Transforming growth factor beta-1-binding protein 1 antibody
    • Transforming growth factor beta-1-masking protein large subunit antibody
    see all

Anti-LTBP1 antibody 画像

  • All lanes : Anti-LTBP1 antibody (ab78294) at 1 µg/ml

    Lane 1 : Liver (Rat) Tissue Lysate
    Lane 2 : Pancreas (Mouse) Tissue Lysate
    Lane 3 : Spleen (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 187 kDa
    Observed band size : 187 kDa
    Based on the immunogen sequence, this antibody is predicted to react with all three isoforms of LTBP1. The band detected here might correspond to the long isoform, which is 187 kDa and is found in fibroblasts.
  • IHC image of LTBP1 staining in Mouse spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78294, 5µg/ml, for 15 mins at room temperature. A Goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-LTBP1 antibody (ab78294) at 1 µg/ml

    Lane 1 : Liver (Rat) Tissue Lysate
    Lane 2 : Spleen (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 187 kDa
    Observed band size : 187 kDa
    Additional bands at : 171 kDa (possible cleavage fragment),55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

Anti-LTBP1 antibody (ab78294) 使用論文

This product has been referenced in:
  • Zhang H  et al. iTRAQ-Based Quantitative Proteomic Comparison of Early- and Late-Passage Human Dermal Papilla Cell Secretome in Relation to Inducing Hair Follicle Regeneration. PLoS One 11:e0167474 (2016). IHC-P . Read more (PubMed: 27907131) »
  • Horiguchi M  et al. Abrogation of both short and long forms of latent transforming growth factor-ß binding protein-1 causes defective cardiovascular development and is perinatally lethal. Matrix Biol 43:61-70 (2015). Read more (PubMed: 25805620) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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