製品の概要

  • 製品名Anti-LRWD1 antibody
    LRWD1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to LRWD1
  • アプリケーション適用あり: WBmore details
    適用なし: IP
  • 種交差性
    交差種: Human
    交差が予測される動物種: Horse, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region between residues 432 and 482 of human LRWD1 (NP_690852.1)

  • ポジティブ・コントロール
    • HeLa and 293T whole cell lysates.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab85738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 機能Associated component of the origin recognition complex (ORC) required to recruit and stabilize the ORC complex to chromatin. Probably required for the association of ORC on chromatin during G1 to establish pre-replication complex (preRC) and to heterochromatic sites in post-replicated cells. Binds a combination of DNA and histone methylation repressive marks on heterochromatin: binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 in a cooperative manner with DNA methylation. It is unclear whether it recongnizes and binds these repressive marks by itself or needs additional factors.
    • 配列類似性Belongs to the LRWD1 family.
      Contains 3 LRR (leucine-rich) repeats.
      Contains 5 WD repeats.
    • ドメインThe WD repeats are required for the interaction with ORC components.
    • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 細胞内局在Nucleus. Chromosome > centromere. Chromosome > telomere. Localizes to heterochromatin during G1 phase. Restricted to centromeres or telomeres as cells progress though S phase. When cells enter mitosis, relocalizes to centromeres.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 1200011O22Rik antibody
      • AU042569 antibody
      • AW548074 antibody
      • DKFZp434K1815 antibody
      • Leucine rich repeats and WD repeat domain containing 1 antibody
      • Leucine-rich repeat and WD repeat-containing protein 1 antibody
      • LRWD1 antibody
      • LRWD1_HUMAN antibody
      • ORC-associated protein antibody
      • ORCA antibody
      • Origin recognition complex-associated protein antibody
      see all

    Anti-LRWD1 antibody 画像

    • All lanes : Anti-LRWD1 antibody (ab85738) at 0.4 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg


      Predicted band size : 71 kDa
      Observed band size : 71 kDa
      Additional bands at : 50 kDa,80 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 30 seconds

    Anti-LRWD1 antibody (ab85738) 使用論文

    ab85738 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab85738.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"