The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform Heat Induced Epitope Retrieval (HIER) at pH 6 using a pressure boiler as heat source.
Renders cells highly sensitive to the activation by cytokines and lipopolysaccharide (LPS).
Expressed at low levels in many tissues, including smooth muscle, brain, uterus, pancreas, cartilage, adipose, spleen and testis.