Anti-LRPPRC/GP130 抗体 (ab97505)
Key features and details
- Rabbit polyclonal to LRPPRC/GP130
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-LRPPRC/GP130 antibody
LRPPRC/GP130 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to LRPPRC/GP130 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat -
免疫原
Recombinant fragment corresponding to Human LRPPRC/GP130 aa 611-907.
Database link: P42704 -
ポジティブ・コントロール
- WB: H1299, HeLa whole cell lysate. IHC-P: SW480 Xenograft, human colon cancer tissue. ICC/IF: HeLa cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97505の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/500 - 1/10000. Predicted molecular weight: 158 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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特記事項 |
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WB
1/500 - 1/10000. Predicted molecular weight: 158 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
May play a role in RNA metabolism in both nuclei and mitochondria. In the nucleus binds to HNRPA1-associated poly(A) mRNAs and is part of nmRNP complexes at late stages of mRNA maturation which are possibly associated with nuclear mRNA export. May bind mature mRNA in the nucleus outer membrane. In mitochondria binds to poly(A) mRNA. Plays a role in translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. May be involved in transcription regulation. Cooperates with PPARGC1A to regulate certain mitochondrially encoded genes and gluconeogenic genes and may regulate docking of PPARGC1A to transcription factors. Seems to be involved in the transcription regulation of the multidrug-related genes MDR1 and MVP. Part of a nuclear factor that binds to the invMED1 element of MDR1 and MVP gene promoters. Binds single-stranded DNA. -
組織特異性
Expressed ubiquitously. Expression is highest in heart, skeletal muscle, kidney and liver, intermediate in brain, non-mucosal colon, spleen and placenta, and lowest in small intestine, thymus, lung and peripheral blood leukocytes. -
関連疾患
Leigh syndrome French-Canadian type -
配列類似性
Contains 20 PPR (pentatricopeptide) repeats. -
細胞内局在
Mitochondrion. Nucleus, nucleoplasm. Nucleus inner membrane. Nucleus outer membrane. Seems to be predominantly mitochondrial. - Information by UniProt
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参照データベース
- Entrez Gene: 10128 Human
- Entrez Gene: 313867 Rat
- Omim: 607544 Human
- SwissProt: P42704 Human
- SwissProt: Q5SGE0 Rat
- Unigene: 368084 Human
- Unigene: 92051 Rat
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別名
- 130 kDa leucine-rich protein antibody
- gp130 antibody
- Leucine-rich PPR motif-containing protein antibody
see all
画像
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All lanes : Anti-LRPPRC/GP130 antibody (ab97505) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 158 kDa
5% SDS-PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed HeLa using ab97505 at 1/200 dilution. Lower panel is also merged with DNA probe.
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Immunohistochemical analysis of paraffin-embedded SW480 Xenograft using ab97505 at 1/100 dilution.
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Paraffin embedded human colon cancer tissue stained for LRP130 using ab97505 at 1/100 at dilution in immunohistochemical analysis.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab97505 は 13 報の論文で使用されています。
- Ke J et al. [Comprehensive Analysis of the Relationship between m6A Methylation Patterns and Immune Microenvironment in Lung Adenocarcinoma]. Zhongguo Fei Ai Za Zhi 25:311-322 (2022). PubMed: 35599007
- Hu Y et al. LRPPRC contributes to the cisplatin resistance of lung cancer cells by regulating MDR1 expression. Oncol Rep 45:N/A (2021). PubMed: 33649818
- Saleem I et al. The Mammalian Ecdysoneless Protein Interacts with RNA Helicase DDX39A To Regulate Nuclear mRNA Export. Mol Cell Biol 41:e0010321 (2021). PubMed: 33941617
- Wurlitzer M et al. Mass Spectrometric Comparison of HPV-Positive and HPV-Negative Oropharyngeal Cancer. Cancers (Basel) 12:N/A (2020). PubMed: 32545200
- Islam H et al. Fiber-specific and whole-muscle LRP130 expression in rested, exercised, and fasted human skeletal muscle. Pflugers Arch 472:375-384 (2020). PubMed: 32065259