製品の概要

  • 製品名Anti-LRPPRC antibody
    LRPPRC 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to LRPPRC
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 950 - 1050 of Human LRPPRC.

    (Peptide available as ab90211.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following human tissue lysates: Heart; Skeletal Muscle; HepG2.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab80881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 155 kDa (predicted molecular weight: 158 kDa).

ターゲット情報

  • 機能May play a role in RNA metabolism in both nuclei and mitochondria. In the nucleus binds to HNRPA1-associated poly(A) mRNAs and is part of nmRNP complexes at late stages of mRNA maturation which are possibly associated with nuclear mRNA export. May bind mature mRNA in the nucleus outer membrane. In mitochondria binds to poly(A) mRNA. Plays a role in translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. May be involved in transcription regulation. Cooperates with PPARGC1A to regulate certain mitochondrially encoded genes and gluconeogenic genes and may regulate docking of PPARGC1A to transcription factors. Seems to be involved in the transcription regulation of the multidrug-related genes MDR1 and MVP. Part of a nuclear factor that binds to the invMED1 element of MDR1 and MVP gene promoters. Binds single-stranded DNA.
  • 組織特異性Expressed ubiquitously. Expression is highest in heart, skeletal muscle, kidney and liver, intermediate in brain, non-mucosal colon, spleen and placenta, and lowest in small intestine, thymus, lung and peripheral blood leukocytes.
  • 関連疾患Leigh syndrome French-Canadian type
  • 配列類似性Contains 20 PPR (pentatricopeptide) repeats.
  • 細胞内局在Mitochondrion. Nucleus, nucleoplasm. Nucleus inner membrane. Nucleus outer membrane. Seems to be predominantly mitochondrial.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 130 kDa leucine-rich protein antibody
    • gp130 antibody
    • Leucine-rich PPR motif-containing protein antibody
    • Leucine-rich PPR-motif containing protein antibody
    • LPPRC_HUMAN antibody
    • LRP 130 antibody
    • lrpprc antibody
    • mitochondrial antibody
    see all

Anti-LRPPRC antibody 画像

  • All lanes : Anti-LRPPRC antibody (ab80881) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 158 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 54 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • ICC/IF image of ab80881 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80881, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2, and MCF-7 cells at 1µg/ml, and in 100% Methanol fixed (5 min) HeLa, Hek293, HepG2, and MCF-7 cells at 1µg/ml

Anti-LRPPRC antibody (ab80881) 使用論文

ab80881 has not yet been referenced specifically in any publications.

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