製品の概要

  • 製品名Anti-LRPAP1 antibody
    LRPAP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to LRPAP1
  • アプリケーション適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human LRPAP1.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates: HEK293 Whole Cell; A498 Whole Cell; Human Brain Tissue; U-87 MG Whole Cell.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab85591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).

ターゲット情報

  • 機能Interacts with LRP1/alpha-2-macroglobulin receptor and glycoprotein 330.
  • 関連疾患Note=In complex with the alpha-2-MR or gp330, it may have some role in the pathogenesis of membrane glomerular nephritis.
  • 配列類似性Belongs to the alpha-2-MRAP family.
  • 翻訳後修飾N-glycosylated.
  • 細胞内局在Endoplasmic reticulum. Cytoplasm. Cell surface. Intracellular and associated with cell surface receptors. Found in the endoplasmic reticulum.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 39 kDa receptor-associated protein antibody
    • A2MRAP antibody
    • A2RAP antibody
    • Alpha 2 macroglobulin receptor associated protein antibody
    • Alpha 2 MRAP antibody
    • Alpha-2-macroglobulin receptor-associated protein antibody
    • Alpha-2-MRAP antibody
    • AMRP_HUMAN antibody
    • HBP44 antibody
    • Lipoprotein receptor associated protein antibody
    • Low density lipoprotein receptor related protein associated protein 1 antibody
    • Low density lipoprotein receptor-related protein-associated protein 1 antibody
    • Low density lipoprotein related protein associated protein 1 alpha 2 macroglobulin receptor associated protein antibody
    • Low density lipoprotein related protein associated protein 1 antibody
    • low density lipoprotein-related protein-associated protein 1 (alpha-2-macroglobulin receptor-associated protein 1) antibody
    • Lrpap1 antibody
    • MGC138272 antibody
    • MRAP antibody
    • MYP23 antibody
    • RAP antibody
    see all

Anti-LRPAP1 antibody 画像

  • All lanes : Anti-LRPAP1 antibody (ab85591) at 1 µg/ml

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
    Lane 3 : Human brain tissue lysate - total protein (ab29466)
    Lane 4 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 41 kDa
    Additional bands at : 37 kDa,50 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • ICC/IF image of ab85591 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85591, µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (10 min) HeLa cells at 5µg/ml.
  • IHC image of LRPAP1 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85591, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-LRPAP1 antibody (ab85591) 使用論文

ab85591 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab85591.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"