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Cytoplasmic domain of human LRP6.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab134146 as a replacement.
Our Abpromise guarantee covers the use of ab75358 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Detects a band of approximately 180 kDa (predicted molecular weight: 179 kDa).|
|IP||1/1000 - 1/2000.|
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: LRP6 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: HepG2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab75358 observed at 200, 220 kDa. Red - loading control, ab18251, observed at 52 kDa.
ab75358 was shown to specifically react with LRP6 when LRP6 knockout samples were used. Wild-type and LRP6 knockout samples were subjected to SDS-PAGE. ab75358 and ab18251 (loading control to alpha Tubulin) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.