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Our Abpromise guarantee covers the use of ab75358 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Detects a band of approximately 180 kDa (predicted molecular weight: 179 kDa).|
|IP||1/1000 - 1/2000.|
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: LRP6 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: HepG2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab75358 observed at 200, 220 kDa. Red - loading control, ab18251, observed at 52 kDa.
ab75358 was shown to specifically react with LRP6 when LRP6 knockout samples were used. Wild-type and LRP6 knockout samples were subjected to SDS-PAGE. ab75358 and ab18251 (loading control to alpha Tubulin) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.