製品の概要

  • 製品名Anti-Lrp2 / Megalin antibody
    Lrp2 / Megalin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Lrp2 / Megalin
  • アプリケーション適用あり: IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human, Monkey
    交差が予測される動物種: Rat, Xenopus laevis
  • 免疫原

    Synthetic peptide derived from within residues 4600 to the C-terminus of Human Lrp2/ Megalin.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human Kidney Carcinoma Tissue (Immunohistochemistry).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Acts together with cubilin to mediate HDL endocytosis (By similarity). May participate in regulation of parathyroid-hormone and para-thyroid-hormone-related protein release.
  • 組織特異性Absorptive epithelia, including renal proximal tubules.
  • 関連疾患Defects in LRP2 are the cause of Donnai-Barrow syndrome (DBS) [MIM:222448]; also known as faciooculoacousticorenal syndrome (FOAR syndrome). DBS is a rare autosomal recessive disorder characterized by major malformations including agenesis of the corpus callosum, congenital diaphragmatic hernia, facial dysmorphology, ocular anomalies, sensorineural hearing loss and developmental delay. The FOAR syndrome was first described as comprising facial anomalies, ocular anomalies, sensorineural hearing loss, and proteinuria. DBS and FOAR were first described as distinct disorders but the classic distinguishing features between the 2 disorders were presence of proteinuria and absence of diaphragmatic hernia and corpus callosum anomalies in FOAR. Early reports noted that the 2 disorders shared many phenotypic features and may be identical. Although there is variability in the expression of some features (e.g. agenesis of the corpus callosum and proteinuria), DBS and FOAR are now considered to represent the same entity.
  • 配列類似性Belongs to the LDLR family.
    Contains 17 EGF-like domains.
    Contains 36 LDL-receptor class A domains.
    Contains 37 LDL-receptor class B repeats.
  • 細胞内局在Membrane. Membrane > coated pit.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Calcium sensor protein antibody
    • DBS antibody
    • Glycoprotein 330 antibody
    • gp330 antibody
    • Heymann nephritis antigen homolog antibody
    • Low-density lipoprotein receptor-related protein 2 antibody
    • LRP-2 antibody
    • Lrp2 antibody
    • LRP2_HUMAN antibody
    • Megalin antibody
    see all

Anti-Lrp2 / Megalin antibody 画像

  • IHC image of ab76969 staining in human kidney carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab76969, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • ab76969 staining Lrp2 / Megalin in monkey kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using citrate pH 6.0. Samples were then incubated with ab76969 at 1µg/ml for 1 hour with BSA in TBS. The secondary used was an undiluted HRP conjugated goat anti-rabbit polyclonal.

    See Abreview

  • ab76969 staining Lrp2 / Megalin in mouse kidney tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, blocked for 60 minutes at 25°C and antigen retrieval was by heat mediation. The sample was incubated with primary antibody (1/1000) at 25°C for 1 hour. An Alexa Fluor® 488-conjugated donkey rabbit polyclonal (1/1200) was used as the secondary antibody.

Anti-Lrp2 / Megalin antibody (ab76969) 使用論文

This product has been referenced in:
  • Burke KA  et al. Expression and immunolocalisation of the endocytic receptors megalin and cubilin in the human yolk sac and placenta across gestation. Placenta 34:1105-9 (2013). Read more (PubMed: 23978537) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (adult and fetal kidney)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: commercial - ZYTOMED's OmniPrep (pH 9)
Permeabilization Yes - 0.05% tween 20 in the washing buffer
Specification adult and fetal kidney
Blocking step commercial - Invitrogen's CAS-Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative Paraformaldehyde
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Abcam user community

Verified customer

投稿 Dec 03 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (mouse kidney)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: commercial - ZYTOMED's OmniPrep (pH 9)
Permeabilization No
Specification mouse kidney
Blocking step commercial - Invitrogen's CAS-Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative Paraformaldehyde
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Abcam user community

Verified customer

投稿 Dec 03 2015

Application Western blot
Sample Rat Tissue lysate - other (Kidney)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification Kidney
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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投稿 Nov 19 2015

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain, ependymal cells (ventricle lining cells))
Antigen retrieval step None
Permeabilization Yes - 0.05% Tween 20
Specification Brain, ependymal cells (ventricle lining cells)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Yi-An Yang

Verified customer

投稿 Nov 13 2015

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Kidney, renal tubular cells)
Permeabilization Yes - 0.05% Tween 20
Specification Kidney, renal tubular cells
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Yi-An Yang

Verified customer

投稿 Nov 12 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Kidney)
Specification Kidney
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH 6.0
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
Username

Rodolfo

Verified customer

投稿 Apr 16 2013

Thank you very much for your interest in ab12228 and ab76969.

To our knowledge, ab12228 or ab76969 has not been tested in Zebrafish. Therefore, I can offer a discount off a future purchase if you buy ab12228 and ab76969 now, test it in Zebra...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Monkey Tissue sections (Kidney)
Specification Kidney
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization No
Username

Abcam user community

Verified customer

投稿 Feb 09 2012

Thank you for contacting us.

We do not have data on how long an antibody can be stored when diluted in swine serum.

The general recommendation would be to store the antibody undiluted as per datasheet instructions (which are for ab...

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Thank you for contacting Abcam regarding ab76969. The tissue specificity information of megalin expression can be found using the SwissProt database: http://www.uniprot.org/uniprot/P98164 The protocol is as follows: - Human kidney carcino...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"