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Synthetic peptide derived from within residues 4600 to the C-terminus of Human Lrp2/ Megalin.
Our Abpromise guarantee covers the use of ab76969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
ab76969 staining Lrp2 / Megalin in mouse kidney tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, blocked for 60 minutes at 25°C and antigen retrieval was by heat mediation. The sample was incubated with primary antibody (1/1000) at 25°C for 1 hour. An Alexa Fluor® 488-conjugated donkey rabbit polyclonal (1/1200) was used as the secondary antibody.