製品の概要
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製品名Anti-LRP1 antibody [8G1]
LRP1 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [8G1] to LRP1
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特異性Specifically reacts with the 515kDa alpha chain of LRP/alpha2 MR expressed in hepatocytes, tissue macrophages, subsets of neurons and astrocytes in the CNS, fibroblasts, smooth muscle cells and monocyte derived foam cells in atherosclerotic lesions of arterial walls.
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アプリケーション適用あり: IHC-Fr, IHC-P, WB, ICC/IF, Electron Microscopy, Dot blot, Flow Cytmore details
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種交差性交差種: Human
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免疫原
Full length protein corresponding to LRP1. J Biol Chem 265:17401-4 (1990).
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: None
Constituents: 50mM Sodium phosphate, 100mM Sodium chloride, pH 7.4 -
Concentration information loading... -
精製度IgG fraction
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ポリ/モノモノクローナル
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クローン名8G1
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アイソタイプIgG1
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軽鎖の種類kappa
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研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
アプリケーション
Our Abpromise guarantee covers the use of ab20384 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr | Use a concentration of 1 - 10 µg/ml. | |
| IHC-P | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 - 5 µg/ml. Use under non reducing condition. | |
| ICC/IF | Use a concentration of 5 µg/ml. | |
| Electron Microscopy | Use at an assay dependent concentration. Use at dilutions of 1/400 or 1/600 for 20 mins. | |
| Dot blot | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能Endocytic receptor involved in endocytosis and in phagocytosis of apoptotic cells. Required for early embryonic development. Involved in cellular lipid homeostasis. Involved in the plasma clearance of chylomicron remnants and activated LRPAP1 (alpha 2-macroglobulin), as well as the local metabolism of complexes between plasminogen activators and their endogenous inhibitors. May modulate cellular events, such as APP metabolism, kinase-dependent intracellular signaling, neuronal calcium signaling as well as neurotransmission.
Functions as a receptor for Pseudomonas aeruginosa exotoxin A. -
組織特異性Most abundant in liver, brain and lung.
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配列類似性Belongs to the LDLR family.
Contains 22 EGF-like domains.
Contains 31 LDL-receptor class A domains.
Contains 34 LDL-receptor class B repeats. -
翻訳後修飾Cleaved into a 85 kDa membrane-spanning subunit (LRP-85) and a 515 kDa large extracellular domain (LRP-515) that remains non-covalently associated. Gamma-secretase-dependent cleavage of LRP-85 releases the intracellular domain from the membrane.
The N-terminus is blocked.
Phosphorylated on serine and threonine residues.
Phosphorylated on tyrosine residues upon stimulation with PDGF. Tyrosine phosphorylation promotes interaction with SHC1. -
細胞内局在Cytoplasm. Nucleus. After cleavage, the intracellular domain (LRPICD) is detected both in the cytoplasm and in the nucleus and Cell membrane. Membrane, coated pit.
- Information by UniProt
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参照データベース
- Entrez Gene: 4035 Human
- Omim: 107770 Human
- SwissProt: Q07954 Human
- Unigene: 162757 Human
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別名
- A2MR antibody
- Alpha 2 macroglobulin receptor antibody
- alpha 2MR antibody
see all
Anti-LRP1 antibody [8G1] 画像
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![Flow Cytometry - Low Density LRP antibody [8G1] (ab20384)](http://a.abcam.com/ps/datasheet/Images/20/ab20384/ab20384_1.jpg)
Flow Cytometry - Low Density LRP antibody [8G1] (ab20384)This image is courtesy of an anonymous Abreviewab20384 staining human mesothelial cells by flow cytometry. Cells were lifted in lifting buffer plus FBS and fixed with formaldehyde. The cell population was gated against mIgG control. The primary antibody was diluted 1/100 in 0.2% BSA in PBS and incubated with the sample at a concentration of 1 µg/100 µl for 1 hour at 4°C. A FITC conjugated goat anti-mouse antibody was used as the secondary, diluted 1/100 and incubated with the sample at a concentration of 1 µg/100 µl.
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![Electron Microscopy - Low Density LRP antibody [8G1] (ab20384)](http://a.abcam.com/ps/datasheet/images/20/ab20384/Low-Density-LRP-Primary-antibodies-ab20384-2.jpg)
Electron Microscopy - Low Density LRP antibody [8G1] (ab20384)Ab20384 staining primary rat hepatocytes cells by EM. The cells were fixed and prepared by Tokuyasu method. The cryosections of 70nm were placed on 100 mesh grids. All the following steps were done at room temperature. Blocking was carried out for 30 min with 1% fish skin gelatine (FSG). The tissue sections were placed on a drop of 5 ul of first Ab (anti-LRP 8G1) for 20 min at dilutions of 1:400 or 1:600 seemed to work the best. After incubation with the primary antibody, 3. 5 x 2 min wash was done with PBS and the sections were placed on a drop of 5 ul of secondary Ab (goat anti-mouse) for 20 min (dilution 1:150 5. 5 x 2 min wash with PBS). Samples were placed on a drop of 5 ul of PAG10 for 15 min (dilution 1:60 or 1:70) and then 5 x 2 min wash with PBS. 2 min on 1% glutaraldehyde for fixation and 4 x 2.5 min on H2O. afterthat 1 second + 1 second + 6 min on drops of Methyl Cellulose/Uranyl Acetate (9:1) on ice. The surplus of MC/UA was removed , the slides were dried and observed at the TEM. This image was kindly provided by our customer. -
](http://a.abcam.com/ps/datasheet/images/20/ab20384/Low-Density-LRP-Primary-antibodies-ab20384-3.jpg)
Immunocytochemistry/ Immunofluorescence-Low Density LRP antibody [8G1](ab20384)ICC/IF image of ab20384 stained Mcf7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20384, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
Anti-LRP1 antibody [8G1] (ab20384) 使用論文
This product has been referenced in:
- Dohler F et al. High molecular mass assemblies of amyloid-ß oligomers bind prion protein in patients with Alzheimer's disease. Brain 137:873-86 (2014). WB ; Human . Read more (PubMed: 24519981) »
- Scilabra SD et al. Differential regulation of extracellular tissue inhibitor of metalloproteinases-3 levels by cell membrane-bound and shed low density lipoprotein receptor-related protein 1. J Biol Chem 288:332-42 (2013). WB . Read more (PubMed: 23166318) »
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