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Our Abpromise guarantee covers the use of ab20384 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use a concentration of 1 - 10 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 5 µg/ml. Use under non reducing condition.|
|ICC/IF||Use a concentration of 5 µg/ml.|
|Electron Microscopy||Use at an assay dependent concentration. Use at dilutions of 1/400 or 1/600 for 20 mins.|
|Dot blot||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
ab20384 staining human mesothelial cells by flow cytometry. Cells were lifted in lifting buffer plus FBS and fixed with formaldehyde. The cell population was gated against mIgG control. The primary antibody was diluted 1/100 in 0.2% BSA in PBS and incubated with the sample at a concentration of 1 µg/100 µl for 1 hour at 4°C. A FITC conjugated goat anti-mouse antibody was used as the secondary, diluted 1/100 and incubated with the sample at a concentration of 1 µg/100 µl.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"