製品の概要

  • 製品名Anti-LRP1 antibody [5A6]
    LRP1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [5A6] to LRP1
  • アプリケーション適用あり: Flow Cyt, WB, IHC-P, IHC-Fr, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Human
  • 免疫原

    Recombinant fragment (Human), 85kDa cleaved fragment of Low Density LRP

  • ポジティブ・コントロール
    • Ab28320 gave a positive signal in Western Blot in WI38 whole cell lysate. This antibody also gave a positive result in IHC in the following FFPE tissue: Human normal placenta.

アプリケーション

Our Abpromise guarantee covers the use of ab28320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 µg/ml. Use under non reducing condition. Detects a band of approximately 80 kDa (predicted molecular weight: 85 kDa).

Abcam do not recommend adding a reducing agent (e.g. DTT) to lysates or heating samples prior to loading on SDS-PAGE gels.  We have used BSA as our blocking agent at 1%.

IHC-P Use a concentration of 10 µg/ml.
IHC-Fr Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能Endocytic receptor involved in endocytosis and in phagocytosis of apoptotic cells. Required for early embryonic development. Involved in cellular lipid homeostasis. Involved in the plasma clearance of chylomicron remnants and activated LRPAP1 (alpha 2-macroglobulin), as well as the local metabolism of complexes between plasminogen activators and their endogenous inhibitors. May modulate cellular events, such as APP metabolism, kinase-dependent intracellular signaling, neuronal calcium signaling as well as neurotransmission.
    Functions as a receptor for Pseudomonas aeruginosa exotoxin A.
  • 組織特異性Most abundant in liver, brain and lung.
  • 配列類似性Belongs to the LDLR family.
    Contains 22 EGF-like domains.
    Contains 31 LDL-receptor class A domains.
    Contains 34 LDL-receptor class B repeats.
  • 翻訳後修飾Cleaved into a 85 kDa membrane-spanning subunit (LRP-85) and a 515 kDa large extracellular domain (LRP-515) that remains non-covalently associated. Gamma-secretase-dependent cleavage of LRP-85 releases the intracellular domain from the membrane.
    The N-terminus is blocked.
    Phosphorylated on serine and threonine residues.
    Phosphorylated on tyrosine residues upon stimulation with PDGF. Tyrosine phosphorylation promotes interaction with SHC1.
  • 細胞内局在Cytoplasm. Nucleus. After cleavage, the intracellular domain (LRPICD) is detected both in the cytoplasm and in the nucleus and Cell membrane. Membrane, coated pit.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A2MR antibody
    • Alpha 2 macroglobulin receptor antibody
    • alpha 2MR antibody
    • Alpha-2-macroglobulin receptor antibody
    • APOER antibody
    • Apolipoprotein E receptor antibody
    • APR antibody
    • CD 91 antibody
    • CD91 antibody
    • CD91 antigen antibody
    • IGFBP3R antibody
    • LDL receptor related protein 1 antibody
    • Low density lipoprotein receptor related protein 1 antibody
    • Low density lipoprotein related protein 1 antibody
    • Low-density lipoprotein receptor-related protein 1 intracellular domain antibody
    • LRP 1 antibody
    • LRP 515 antibody
    • LRP 85 antibody
    • LRP antibody
    • LRP ICD antibody
    • LRP-1 antibody
    • LRP-515 antibody
    • LRP-85 antibody
    • Lrp1 antibody
    • LRP1 protein antibody
    • LRP1_HUMAN antibody
    • LRP1A antibody
    • LRP515 antibody
    • LRP85 antibody
    • LRPICD antibody
    • MGC88725 antibody
    • Prolow density lipoprotein receptor related protein 1 antibody
    • TbetaR V/LRP 1/IGFBP 3 receptor antibody
    • TbetaRV/LRP1/IGFBP3 receptor antibody
    • TGFBR 5 antibody
    • TGFBR5 antibody
    • Type V tgf beta receptor antibody
    see all

Anti-LRP1 antibody [5A6] 画像

  • Anti-LRP1 antibody [5A6] (ab28320) at 1 µg/ml + WI38 (Human lung fibroblast cell line) Whole Cell Lysate [Non-reduced] at 25 µg

    Secondary
    Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/10000 dilution
    Developed using the ECL technique

    Predicted band size : 85 kDa
    Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 1% Bovine Serum Albumin before being incubated with ab28320 overnight at 4°C.  Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution.  Please note that no reducing agent was added to the cell lysate and the sample was not heated prior to loading onto the SDS-PAGE gel. 

  • IHC image of LRP1 staining in Human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab28320, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Overlay histogram showing HepG2 cells stained with ab28320 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28320, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a significantly decreased signal in HepG2 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

    Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

  • ab28320 at 1/100 staining human HUVEC cells by ICC/IF. The cells were methanol fixed and blocked with BSA prior to incuabtion with the antibody for 1 hour. A FITC conjugated goat anti-mouse IgG antibody was used as the secondary.

    See Abreview

  • ab28320 at 1/3000 + WI38 fibroblasts whole cell lysate

    Secondary
    HRP conjugated donkey anti-mouse polyclonal diluted 1/10000
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Predicted band size : 85 kDa
    Observed band size : 85 kDa


    Exposure time : 2 minutes

    This image is courtesy of an Abreview submitted by Dr Torry Tucker

    50µg of human fibroblast whole cell lysate were loaded on to the gel and run under denaturing conditions.

    The membrane was blocked with 1% BSA for 1 hour at RT.

    The primary antibody was incubated for 12 hours at 4°C.

    See Abreview

  • ICC/IF image of LRP1 staining in rat mixed glia culture using ab28320 (1:500). The cells were fixed uisng paraformaldehyde and permeabilised using 0.3% TritonX in 0.1%PBS. The cells were then blocked using 10% donkey serum for 1 hour at 24°C. the Cells were then inubated with ab23020 for 4 hours at 24°C. The secondary antibody used was donkey polyclonal to anti mouse IgG conjuagted to Alexa Fluor 568.

    See Abreview

Anti-LRP1 antibody [5A6] (ab28320) 使用論文

This product has been referenced in:
  • Durk MR  et al. 1a,25-Dihydroxyvitamin D3 reduces cerebral amyloid-ß accumulation and improves cognition in mouse models of Alzheimer's disease. J Neurosci 34:7091-101 (2014). Mouse . Read more (PubMed: 24849345) »
  • Cao C  et al. Placental heme receptor LRP1 correlates with the heme exporter FLVCR1 and neonatal iron status. Reproduction 148:295-302 (2014). Human . Read more (PubMed: 24947444) »

See all 12 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Mixed glial culture)
Specification Mixed glial culture
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Dec 11 2012

Thank you for getting back to me.

Your order number for your free of charge replacment antibody ab92544 is 1210558. You should be receiving the antibody tomorrow. Also, for your own records, it appears the PO number used for the original order...

Read More


I now have answer from the producing lab of ab28320. I can confirm that Immunogen is the 85kDa cleaved fragment of Low Density LRP and therefore it is not strictly c-terminal.
I hope the other antibody that binds a c-terminal epitope ab92544 ...

Read More

Please excuse the delay in my answer.
I am still waiting to hear back from the producing lab concerning the immunogen of ab28320.
But I am happy to confirm that ab92544 (Anti-LRP1 antibody [EPR3724]) binds to c-terminal (cytoplasmic) part of ...

Read More

I hope you are well.
I am happy to confirm that the current lot in stock of ab28320 has a concentration of 7.6mg/ml.
I hope this information is helpful and wish you a good week.

Thank you for contacting Abcam and I do apologize for the delay in this reply.

I don't have a specific protocol for flow cytometry. We do have FACS dilution suggested at 1:1500. I have also attached the general Abcam protocols booklet, which c...

Read More

Thank you for your phone call.

DISCOUNT CODE: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab28320 in rat. This code will give you: 1 free PRIMARY ANTIBODYbefore the expiration...

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Thank you very much for your interest in LRP1 antibodies for use in neutralizing assays.

As I had mentyioned on the phone, we have not yet tested any of our anti-LRP1 antibodies in this application. As such these would qualify for participat...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization No
Blocking step Serum as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Nov 08 2011

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (mouse lung)
Specification mouse lung
Fixative Methanol
Permeabilization Yes - 0.1% Triton-X100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C
Username

Abcam user community

Verified customer

投稿 Jun 13 2011

1-10 of 14 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"