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Our Abpromise guarantee covers the use of ab36707 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 155 kDa (predicted molecular weight: 143 kDa).Can be blocked with LRIG1 peptide (ab207566).|
|IHC-P||Use at an assay dependent concentration.|
ab36707 at 1/200 staining human anal cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step was performed in citrate buffer. The tissue was blocked with serum and then incubated with the antibody for 45 minutes at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.