製品の概要

  • 製品名Anti-LRIG1 antibody
    LRIG1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to LRIG1
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide (human) conjugated to KLH. The peptide corresponded to part of the cytoplasmic tail (C-terminal), sequence is proprietary.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab36707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 155 kDa (predicted molecular weight: 143 kDa).Can be blocked with LRIG1 peptide (ab207566).
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能Act as a feedback negative regulator of signaling by receptor tyrosine kinases, through a mechanism that involves enhancement of receptor ubiquitination and accelerated intracellular degradation.
  • 組織特異性Widely expressed.
  • 配列類似性Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 15 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
  • ドメインThe LRRs and the Ig-domains are each sufficient for EGFR/ERBB1 binding. This interaction is abolished only when these two domains are deleted.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Leucine rich repeat protein antibody
    • Leucine rich repeat protein LRIG1 antibody
    • leucine rich repeats and immunoglobulin like domains 1 antibody
    • Leucine-rich repeats and immunoglobulin-like domains protein 1 antibody
    • leucine-rich repeats- and immunoglobulin-like domains-cotnaining protein 1 antibody
    • LIG 1 antibody
    • LIG-1 antibody
    • LIG1 antibody
    • Lrig1 antibody
    • LRIG1_HUMAN antibody
    • Ortholog of mouse integral membrane glycoprotein LIG 1 antibody
    see all

Anti-LRIG1 antibody 画像

  • ab36707 at 1/200 staining human anal cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step was performed in citrate buffer. The tissue was blocked with serum and then incubated with the antibody for 45 minutes at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • ab36707 staining LRIG1 in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C. No antigen retrieval was performed. Samples were incubated with primary antibody (1/400 in PBS) for 12 hours at 4°C. A Biotin-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Anti-LRIG1 antibody (ab36707) at 1 µg/ml + Human colon tissue lysate - total protein (ab30051) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 143 kDa
    Observed band size : 155 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

Anti-LRIG1 antibody (ab36707) 使用論文

This product has been referenced in:
  • Deng ZY  et al. Investigation of the association between miR-181b, Bcl-2 and LRIG1 in oral verrucous carcinoma. Mol Med Rep 14:2991-6 (2016). IHC ; Human . Read more (PubMed: 27509922) »
  • Oh YM  et al. USP8 modulates ubiquitination of LRIG1 for Met degradation. Sci Rep 4:4980 (2014). WB ; Human . Read more (PubMed: 24828152) »

See all 2 Publications for this product

Product Wall

Thank you very much for your call today and for letting us know about the trouble with ab36707.

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Thank you for contacting us. I can confirm the following information:

- The antibody is affinity purified against the immunizing peptide, therefore all antibodies present will bind this sequence.
- The LRIG1 peptide used to generate thi...

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Thank you for your call on Friday and for your interest in ab36707.

As we discussed, we fully guarantee this antibody to work in Western blot and IHC-P with mouse samples. Should you have any trouble using ab36707 in these applications, pleas...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Sep 14 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (anal cancer)
Specification anal cancer
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Nov 02 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"