製品の概要

  • 製品名Anti-LRG1 antibody
    LRG1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to LRG1
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Macaque Monkey, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human LRG1.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following tissue lysates: Human Liver; Human Plasma Total protein; Human Spleen; Human Small Intestine; Human Lung. IHC-P: FFPE human liver tissue sections.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab63836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 38 kDa).

ターゲット情報

  • 組織特異性Plasma.
  • 配列類似性Contains 8 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1300008B03Rik antibody
    • 2310031E04Rik antibody
    • A2GL antibody
    • A2GL_HUMAN antibody
    • HMFT1766 antibody
    • Leucine rich alpha 2 glycoprotein antibody
    • Leucine-rich alpha-2-glycoprotein 1 antibody
    • Leucine-rich alpha-2-glycoprotein antibody
    • Leucine-rich alpha-2-glycoprotein precursor antibody
    • LRG antibody
    • LRG1 antibody
    see all

Anti-LRG1 antibody 画像

  • IHC image of LRG1 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab63836, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-LRG1 antibody (ab63836) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Human Plasma Total Protein Lysate
    Lane 3 : Human spleen tissue lysate - total protein (ab29699)
    Lane 4 : Human small intestine tissue lysate - total protein (ab29276)
    Lane 5 : Lung (Human) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
    LRG1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

Anti-LRG1 antibody (ab63836) 使用論文

ab63836 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.

I have had a look at all the anti-LRG1 antibodies we have to see if any of them might be able to pick up this difference in glycosylation.

The only one I would suggest might be worth trying is the ab63836. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"