製品の概要

  • 製品名
    Anti-LRDD antibody
  • 製品の詳細
    Rabbit polyclonal to LRDD
  • 特異性
    Based on the immunogen information this product will recognise isoforms 1, 2, 3 and 4 of human LRDD.
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 850 to the C-terminus of Human LRDD.

    (Peptide available as ab87817.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in human liver tissue lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab78389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 99.7 kDa (predicted molecular weight: 99.7 kDa).

ターゲット情報

  • 関連性
    The death domain (DD) containing protein PIDD is a p53 target gene in an erythroleukemia cell line that undergoes G1 phase arrest and subsequent apoptosis after p53 expression. Independently, PIDD was also described as a DD-containing protein with unknown function. The N-terminal region of PIDD contains seven leucine-rich repeats (LRRs), a protein interaction motif found in various proteins with diverse functions, followed by two ZU-5 domains and a C-terminal DD. PIDD forms a complex with caspase-2 and the adaptor protein RAIDD. Increased PIDD expression results in spontaneous activation of caspase-2 and sensitization to apoptosis by genotoxic stimuli, via interaction with caspase-2 and CRADD/RAIDD. PIDD also promotes apoptosis downstream of p53 as component of the DNA damage/stress response pathway that connects p53/TP53 to apoptosis. PIDD has also been shown to interact with NEMO/IKBKG and RIP1 and enhance sumoylation and ubiquitination of NEMO/IKBKG, an important component for activation of the transcription factor NF-kappa-B.
  • 細胞内局在
    Cytoplasm. Nucleus.
  • 参照データベース
  • 別名
    • DKFZp434D229 antibody
    • Leucine rich repeats and death domain containing antibody
    • Leucine-rich repeats and death domain containing antibody
    • MGC16925 antibody
    • p53-induced protein with a death domain antibody
    • PIDD antibody
    see all

画像

  • Anti-LRDD antibody (ab78389) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 99.7 kDa
    Observed band size : 99 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 33 kDa (possible non-specific binding),37 kDa (possible non-specific binding),66 kDa (possible isoform).

    Exposure time : 3 minutes
  • ICC/IF image of ab78389 stained MCF-7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78389, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, HEK293, HepG2 cells at 1µg/ml, and in 100% Methanol fixed (5 min) HepG2, and MCF-7 cells at 1µg/ml.

参考文献

This product has been referenced in:
  • Klinck R  et al. Alternative splicing in osteoclasts and Paget's disease of bone. BMC Med Genet 15:98 (2014). WB, ICC/IF ; Human . Read more (PubMed: 25115182) »
  • Lin J  et al. The calcium-binding protein S100B down-regulates p53 and apoptosis in malignant melanoma. J Biol Chem 285:27487-98 (2010). WB ; Human . Read more (PubMed: 20587415) »

See all 2 Publications for this product

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