Anti-PIDD1 抗体 (ab78389)
Key features and details
- Rabbit polyclonal to PIDD1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PIDD1 antibody
PIDD1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PIDD1 -
由来種
Rabbit -
特異性
Based on the immunogen information this product will recognise isoforms 1, 2, 3 and 4 of human PIDD1. From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Monkey -
免疫原
Synthetic peptide corresponding to Human PIDD1 aa 850 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab87817) -
ポジティブ・コントロール
- This antibody gave a positive signal in human liver tissue lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab78389の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 1 µg/ml.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 99.7 kDa (predicted molecular weight: 99.7 kDa).
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特記事項 |
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ICC/IF
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 99.7 kDa (predicted molecular weight: 99.7 kDa). |
ターゲット情報
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関連性
The death domain (DD) containing protein PIDD is a p53 target gene in an erythroleukemia cell line that undergoes G1 phase arrest and subsequent apoptosis after p53 expression. Independently, PIDD was also described as a DD-containing protein with unknown function. The N-terminal region of PIDD contains seven leucine-rich repeats (LRRs), a protein interaction motif found in various proteins with diverse functions, followed by two ZU-5 domains and a C-terminal DD. PIDD forms a complex with caspase-2 and the adaptor protein RAIDD. Increased PIDD expression results in spontaneous activation of caspase-2 and sensitization to apoptosis by genotoxic stimuli, via interaction with caspase-2 and CRADD/RAIDD. PIDD also promotes apoptosis downstream of p53 as component of the DNA damage/stress response pathway that connects p53/TP53 to apoptosis. PIDD has also been shown to interact with NEMO/IKBKG and RIP1 and enhance sumoylation and ubiquitination of NEMO/IKBKG, an important component for activation of the transcription factor NF-kappa-B. -
細胞内局在
Cytoplasm. Nucleus. -
参照データベース
- Entrez Gene: 55367 Human
- Omim: 605247 Human
- SwissProt: Q9HB75 Human
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別名
- DKFZp434D229 antibody
- Leucine rich repeats and death domain containing antibody
- Leucine-rich repeats and death domain containing antibody
see all
画像
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Anti-PIDD1 antibody (ab78389) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 99.7 kDa
Observed band size: 99 kDa why is the actual band size different from the predicted?
Additional bands at: 33 kDa (possible non-specific binding), 37 kDa (possible non-specific binding), 66 kDa (possible isoform)
Exposure time: 3 minutesPIDD1 has seven different isoforms (SwissProt) which all have differing molecular weights. The image shows the primary target (Isoform 1) at 99 kDa as well as isoform 4 (66 kDa).
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ICC/IF image of ab78389 stained MCF-7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78389, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, HEK293, HepG2 cells at 1µg/ml, and in 100% Methanol fixed (5 min) HepG2, and MCF-7 cells at 1µg/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab78389 は 3 報の論文で使用されています。
- Yao TH et al. Propranolol induces hemangioma endothelial cell apoptosis via a p53-BAX mediated pathway. Mol Med Rep 18:684-694 (2018). PubMed: 29767244
- Klinck R et al. Alternative splicing in osteoclasts and Paget's disease of bone. BMC Med Genet 15:98 (2014). WB, ICC/IF ; Human . PubMed: 25115182
- Lin J et al. The calcium-binding protein S100B down-regulates p53 and apoptosis in malignant melanoma. J Biol Chem 285:27487-98 (2010). WB ; Human . PubMed: 20587415