製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to LPCAT1
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Chicken, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to an internal region within amino acids 324-373 (

    RLPADTCLLEFARLVRGLGLKPEKLEKDLDRYSERARMKGGEKIGIAEFA

    ) of Human LPCAT1 (NP_079106)

  • ポジティブ・コントロール
    • Human fetal small intestine lysate IF/ICC: A549 cell line.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG

アプリケーション

Our Abpromise guarantee covers the use of ab94903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 59 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Possesses both acyltransferase and acetyltransferase activities. Activity is calcium-independent. Mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC). Displays a clear preference for saturated fatty acyl-CoAs, and 1-myristoyl or 1-palmitoyl LPC as acyl donors and acceptors, respectively. May synthesize phosphatidylcholine in pulmonary surfactant, thereby playing a pivotal role in respiratory physiology.
  • パスウェイ
    Lipid metabolism; phospholipid metabolism.
  • 配列類似性
    Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.
    Contains 2 EF-hand domains.
  • ドメイン
    The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphocholine.
    The di-lysine motif confers endoplasmic reticulum localization for type I membrane proteins.
  • 細胞内局在
    Endoplasmic reticulum membrane. Golgi apparatus membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1 acylglycerophosphocholine O acyltransferase antibody
    • 1 alkylglycerophosphocholine O acetyltransferase antibody
    • 1-acylglycerophosphocholine O-acyltransferase antibody
    • 1-alkylglycerophosphocholine O-acetyltransferase antibody
    • Acetyl CoA:lyso PAF acetyltransferase antibody
    • Acetyl CoA:lyso platelet activating factor acetyltransferase antibody
    • Acetyl-CoA:lyso-PAF acetyltransferase antibody
    • Acetyl-CoA:lyso-platelet-activating factor acetyltransferase antibody
    • Acyl CoA:lysophosphatidylcholine acyltransferase 1 antibody
    • Acyltransferase like 2 antibody
    • Acyltransferase-like 2 antibody
    • AGPAT10 antibody
    • AGPAT9 antibody
    • AYTL1 antibody
    • AYTL2 antibody
    • FLJ12443 antibody
    • FLJ41609 antibody
    • LPC acyltransferase 1 antibody
    • LPC acyltransferase antibody
    • lpcat antibody
    • LPCAT-1 antibody
    • LPCAT1 antibody
    • Lyso PAF acetyltransferase antibody
    • Lyso-PAF acetyltransferase antibody
    • LysoPAFAT antibody
    • LysoPC acyltransferase 1 antibody
    • Lysophosphatidylcholine acyltransferase 1 antibody
    • PCAT1_HUMAN antibody
    • PFAAP3 antibody
    • Phosphonoformate immuno associated protein 3 antibody
    • Phosphonoformate immuno-associated protein 3 antibody
    see all

画像

  • ICC/IF image of ab94903 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab94903, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • Anti-LPCAT1 antibody (ab94903) at 1 µg/ml + Human fetal small intestine lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 59 kDa

参考文献

This product has been referenced in:
  • Wu H  et al. Hypoxia-mediated impaired erythrocyte Lands' Cycle is pathogenic for sickle cell disease. Sci Rep 6:29637 (2016). WB ; Mouse, Human . Read more (PubMed: 27436223) »
  • Gao L  et al. Steroid receptor coactivators 1 and 2 mediate fetal-to-maternal signaling that initiates parturition. J Clin Invest 125:2808-24 (2015). Read more (PubMed: 26098214) »

See all 2 Publications for this product

レビューと Q&A

Thank you for your reply.

Regarding a positive control, I would like to recommend the following:

ab29277 - Small intestine (Human) Whole Cell Lysate - fetal normal tissue
ab30203 - Kidney (Human) Tissue Lysate - adult normal ...

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I am very pleased to hear you would like to accept our offer and test ab94903 in rat in WB. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for rat in WB and include this code ...

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Thank you very much for your interest in ab94903.

To our knowledge, ab94903 has not been tested in rat in WB. Therefore, I can offer a discount off a future purchase if you buy ab94903 now, test it in rat in WB and submit feedback to us in t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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