Customer reviews and FAQs

製品の概要

  • 製品名
    Anti-LOK antibody
    LOK 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to LOK
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Human LOK aa 692-721 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: O94804

  • ポジティブ･コントロール
    • WB: Jurkat cell lysate. IHC-P: Human hepatocarcinoma tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項（精製）
    This antibody is purified through a protein A column, followed by peptide affinity purification.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab37727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 112 kDa.
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能
    Can act on substrates such as myelin basic protein and histone 2A on serine and threonine residues.
  • 組織特異性
    Expressed predominantly in lymphoid organs.
  • 配列類似性
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾
    Autophosphorylated.
  • Information by UniProt
  • 参照データベース
    • 別名
      • LOK antibody
      • Lymphocyte oriented kinase antibody
      • Lymphocyte-oriented kinase antibody
      • PRO2729 antibody
      • Serine threonine protein kinase 10 antibody
      • Serine/threonine-protein kinase 10 antibody
      • STK10 antibody
      • STK10_HUMAN antibody
      see all

    Anti-LOK antibody 画像

    • Western blot - LOK antibody - Carboxyterminal end (ab37727)
      Western blot - LOK antibody - Carboxyterminal end (ab37727)
      Anti-LOK antibody (ab37727) + Jurkat cell lysate

      Predicted band size : 112 kDa
      Observed band size : 112 kDa
      Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOK antibody (ab37727)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOK antibody (ab37727)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocarcinoma tissue labelling LOK with ab37727. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by DAB staining.

    Anti-LOK antibody (ab37727) 使用論文

    ab37727 has not yet been referenced specifically in any publications.

    ab37727 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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    ab37727 の abreview を投稿する ab37727 に関する問い合わせをする

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    Western blot abreview for Anti-LOK antibody - Carboxyterminal end

     Average
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (lymphoblast cells +/+ and +/- Stk10)
    Loading amount
    25 µg
    Specification
    lymphoblast cells +/+ and +/- Stk10
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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    Verified customer

    投稿 Feb 26 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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