製品の概要

  • 製品名Anti-Lnx1 antibody
    Lnx1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to Lnx1
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Recombinant Fragment
    交差が予測される動物種: Mouse, Cow, Human
  • 免疫原

    Recombinant fragment: DNVGNLHFLY SELCKGAFHY GLTKDRKRRS QDGCPDGCAS LTATAPSPEV SAAATISLMT DEPGLDNPAY VSSAEDGQPA ISPVDSGRSN RTRARPFERS , corresponding to amino acids 19-119 of human Lnx1 with a tag.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報ELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 81 kDa.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性Lnx1 is a membrane-bound protein that is involved in signal transduction and protein interactions. Lnx1 is an E3 Ubiquitin ligase protein that mediates ubiquitination and subsequent proteasomal degradation of proteins containing phosphotyrosine binding (PTB) domains. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Lnx1 mediates ubiquitination of isoform p66 and isoform p72 of NUMB, but not that of isoform p71 or isoform p65.
    • 細胞内局在Cytoplasmic
    • 参照データベース
    • 別名
      • E3 ubiquitin protein ligase LNX antibody
      • Ligand of Numb protein X 1 antibody
      • Ligand of numb protein X 1 E3 ubiquitin protein ligase antibody
      • LNX antibody
      • LNX1 antibody
      • MPDZ antibody
      • Multi PDZ domain containing protein E3 ubiquitin protein ligase LNX antibody
      • Numb binding protein 1 antibody
      • PDZ domain-containing ring finger protein 2 antibody
      • PDZRN2 antibody
      see all

    Anti-Lnx1 antibody 画像

    • Anti-Lnx1 antibody (ab76716) at 5 µg/ml + immunogen at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

      Predicted band size : 81 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)

    Anti-Lnx1 antibody (ab76716) 使用論文

    This product has been referenced in:
    • Guo Z  et al. Proteomics strategy to identify substrates of LNX, a PDZ domain-containing E3 ubiquitin ligase. J Proteome Res 11:4847-62 (2012). Read more (PubMed: 22889411) »

    See 1 Publication for this product

    Product Wall

    Thank you for your reply and for confirming that.

    I have now arranged for ab140793 to be shipped to you. This will not be shipped to you until the 2nd of January so you can expect to receive it on the 3rd on the order number 1226775. Please le...

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    Thank you for your reply and I am sorry for the delay in mine.

    I am sorry to hear even with further optimisation, you are still not seeing any bands with this antibody. We have as yet only tried this antibody with a recombinantly expressed fra...

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    Thank you for contacting us.

    I am sorry to hear you are experiencing difficulties with the anti-Lnx1 antibody (ab76716). We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"