製品の概要

  • 製品名Anti-LMW Kininogen antibody
    LMW Kininogen 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to LMW Kininogen
  • アプリケーション適用あり: ICC/IF, WB, IP, ELISA, RIA, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Human Kininogen (LMW) purified from human plasma

  • ポジティブ・コントロール
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal liver.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab79650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 20 µg/ml. Predicted molecular weight: 48 kDa.
IP Use at an assay dependent concentration.
ELISA Use a concentration of 4 - 10 µg/ml.
RIA Use a concentration of 4 - 10 µg/ml.
IHC-P Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能(1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.
  • 組織特異性Secreted in plasma. T-kinin is detected in malignant ovarian, colon and breast carcinomas, but not in benign tumors.
  • 関連疾患Defects in KNG1 are the cause of high molecular weight kininogen deficiency (HMWK deficiency) [MIM:228960]. HMWK deficiency is an autosomal recessive coagulation defect. Patients with HWMK deficiency do not have a hemorrhagic tendency, but they exhibit abnormal surface-mediated activation of fibrinolysis.
  • 配列類似性Contains 3 cystatin kininogen-type domains.
  • 翻訳後修飾Bradykinin is released from kininogen by plasma kallikrein.
    Hydroxylation of Pro-383 occurs prior to the release of bradykinin.
    Phosphorylation sites are present in the extracellular medium.
    N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
  • 細胞内局在Secreted > extracellular space.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alpha-2-thiol proteinase inhibitor antibody
    • BDK antibody
    • BK antibody
    • Fitzgerald factor antibody
    • High molecular weight kininogen antibody
    • HMWK antibody
    • Ile-Ser-Bradykinin antibody
    • Kallidin I antibody
    • Kallidin II antibody
    • Kininogen 1 antibody
    • KNG antibody
    • KNG1 antibody
    • KNG1_HUMAN antibody
    • LMW antibody
    • Low molecular weight growth-promoting factor antibody
    • Low molecular weight kininogen antibody
    • Williams-Fitzgerald-Flaujeac factor antibody
    see all

Anti-LMW Kininogen antibody 画像

  • ICC/IF image of ab79650 stained HepG2 cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79650, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-LMW Kininogen antibody (ab79650) at 1 µg/ml + Natural Human LMW Kininogen protein (ab91118) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds
  • IHC image of LMW Kininogen staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79650, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-LMW Kininogen antibody (ab79650) 使用論文

This product has been referenced in:
  • Kashuba E  et al. Proteomic analysis of B-cell receptor signaling in chronic lymphocytic leukaemia reveals a possible role for kininogen. J Proteomics 91C:478-485 (2013). Read more (PubMed: 23938224) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Healthy and CLL B-cells)
Loading amount 40 µg
Specification Healthy and CLL B-cells
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Elena Kashuba

Verified customer

投稿 Apr 10 2013

I can confirm that this particular antibody is sourced and tested externally. They have done IP with cell cultures over expressing the LMW Kininogen protein. Therefore, I am sorry they do not have any data to show how it perform in the other samples. <...

Read More

Please check flow cytometry protocol on our website http://www.abcam.com/index.html?pageconfig=resource&rid=11380 The ab79650 is not a labelled antibody so the following procedure will better fit http://www.abcam.com/index.html?pageconfig=resource&...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Daudi cells, lung cancer A549)
Loading amount 20 µg
Specification Daudi cells, lung cancer A549
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Blocking step Milk as blocking agent for 5 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Aug 19 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"