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RabMAb

Anti-LMO2 抗体 [EP3257] (ab91652)

製品の概要

  • 製品名
    Anti-LMO2 antibody [EP3257]
    LMO2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP3257] to LMO2
  • アプリケーション
    適用あり: WB, IP, Flow Cytmore details
    適用なし: ICC or IHC-P
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (Human)

  • ポジティブ・コントロール
    • Raji cell lysate and Fetal kidney lysate
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Concentration information loading...
  • ポリ/モノ
    モノクローナル
  • クローン名
    EP3257
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab91652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
Flow Cyt
  • 追加情報
    Flow Cyt: 1/100 - 1/500.


    IP: 1/100 - 1/500.
    WB: 1/1000 - 1/10000. Predicted molecular weight: 18 kDa.

    Is unsuitable for ICC or IHC-P.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Acts with TAL1/SCL to regulate red blood cell development. Also acts with LDB1 to maintain erythroid precursors in an immature state.
    • 関連疾患
      Note=A chromosomal aberration involving LMO2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(11,14)(p13;q11) with TCRD.
    • 配列類似性
      Contains 2 LIM zinc-binding domains.
    • ドメイン
      The second LIM zinc-binding domain interacts with KDM5A.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Cysteine rich protein TTG 2 antibody
      • Cysteine rich protein TTG2 antibody
      • Cysteine-rich protein TTG-2 antibody
      • LIM domain only 2 (rhombotin like 1) antibody
      • LIM domain only 2 antibody
      • LIM domain only protein 2 antibody
      • LMO 2 antibody
      • LMO-2 antibody
      • lmo2 antibody
      • RBTN 2 antibody
      • RBTN L1 antibody
      • RBTN2 antibody
      • RBTN2_HUMAN antibody
      • RBTNL 1 antibody
      • RBTNL1 antibody
      • RHOM 2 antibody
      • RHOM2 antibody
      • Rhombotin 2 antibody
      • Rhombotin like 1 antibody
      • Rhombotin-2 antibody
      • Rhombotin2 antibody
      • T cell translocation gene 2 antibody
      • T cell translocation protein 2 antibody
      • T-cell translocation protein 2 antibody
      • TTG 2 antibody
      • TTG2 antibody
      see all

    画像

    • Overlay histogram showing Ramos cells stained with ab91652 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab91652, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • All lanes : Anti-LMO2 antibody [EP3257] (ab91652) at 1/1000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : Fetal kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 18 kDa

    参考文献

    This product has been referenced in:
    • Liu Y  et al. LMO2 Enhances Lamellipodia/Filopodia Formation in Basal-Type Breast Cancer Cells by Mediating ARP3-Profilin1 Interaction. Med Sci Monit 23:695-703 (2017). Read more (PubMed: 28170369) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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