製品の概要

  • 製品名Anti-LMCD1 antibody
    LMCD1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to LMCD1
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen sequence, corresponding to amino acids 160-293 of Human LMCD1.

  • ポジティブ・コントロール
    • Human cerebellum tissue; RT-4, U-251 MG cell lysate; Human Liver and Tonsil lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab121788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Transcriptional cofactor that restricts GATA6 function by inhibiting DNA-binding, resulting in repression of GATA6 transcriptional activation of downstream target genes. Represses GATA6-mediated trans activation of lung- and cardiac tissue-specific promoters. Inhibits DNA-binding by GATA4 and GATA1 to the cTNC promoter (By similarity). Plays a critical role in the development of cardiac hypertrophy via activation of calcineurin/nuclear factor of activated T cells signaling pathway.
  • 組織特異性Expressed in the heart (at protein level). Expressed in many tissues with highest abundance in skeletal muscle.
  • 配列類似性Contains 2 LIM zinc-binding domains.
    Contains 1 PET domain.
  • ドメインThe LIM zinc-binding domains and the Cys-rich region mediate interaction with GATA6.
  • 細胞内局在Cytoplasm. Nucleus. May shuttle between the cytoplasm and the nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Dyxin antibody
    • LIM and cysteine rich domains 1 antibody
    • LIM and cysteine rich domains protein 1 antibody
    • LIM and cysteine-rich domains protein 1 antibody
    • LMCD1 antibody
    • LMCD1_HUMAN antibody
    see all

Anti-LMCD1 antibody 画像

  • Immunofluorescent staining of Human cell line A-431 shows positivity in plasma membrane and cell junctions. Recommended concentration of ab121788 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-LMCD1 antibody (ab121788) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human Plasma
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

    Developed using the ECL technique
  • ab121788, at 1/400 dilution, staining LMCD1 in paraffin-embedded Human cerebellum tissue, by Immunohistochemistry.

Anti-LMCD1 antibody (ab121788) 使用論文

ab121788 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab121788.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"