製品の概要

  • 製品名Anti-LMAN1 antibody [EPR6979]
    LMAN1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR6979] to LMAN1
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to residues in Human LMAN1.

  • ポジティブ・コントロール
    • HeLa, 293T, SH-SY5Y, NCI-H460, Jurkat and JAR cell lysates; Human kidney tissue and Human papillary carcinoma of thyroid gland tissues. This antibody gave a positive result when used in the following methanol fixed cell lines: HeLa
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab125006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/50 - 1/100.
WB 1/1000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 58 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
  • 追加情報Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins.
    • 組織特異性Ubiquitous.
    • 関連疾患Defects in LMAN1 are THE cause of factor V and factor VIII combined deficiency type 1 (F5F8D1) [MIM:227300]; also known as multiple coagulation factor deficiency I (MCFD1). F5F8D1 is an autosomal recessive blood coagulation disorder characterized by bleeding symptoms similar to those in hemophilia or parahemophilia, that are caused by single deficiency of FV or FVIII, respectively. The most common symptoms are epistaxis, menorrhagia, and excessive bleeding during or after trauma. Plasma levels of coagulation factors V and VIII are in the range of 5 to 30% of normal.
    • 配列類似性Contains 1 L-type lectin-like domain.
    • 翻訳後修飾The N-terminal may be partly blocked.
    • 細胞内局在Endoplasmic reticulum-Golgi intermediate compartment membrane. Golgi apparatus membrane. Endoplasmic reticulum membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Endoplasmic reticulum golgi intermediate compartment protein 53 antibody
      • ER-Golgi intermediate compartment 53 kDa protein antibody
      • ERGIC-53 antibody
      • ERGIC53 antibody
      • ERGIC53 like protein antibody
      • F5F8D antibody
      • FMFD1 antibody
      • Gp58 antibody
      • Intracellular mannose specific lectin antibody
      • Intracellular mannose-specific lectin MR60 antibody
      • Lectin mannose binding 1 antibody
      • Lectin mannose-binding 1 antibody
      • Lman1 antibody
      • LMAN1 like protein antibody
      • LMAN1_HUMAN antibody
      • MCFD1 antibody
      • MR60 antibody
      • Protein ERGIC-53 antibody
      see all

    Anti-LMAN1 antibody [EPR6979] 画像

    • ICC/IF image of ab125006 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab125006 at 1/100 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • All lanes : Anti-LMAN1 antibody [EPR6979] (ab125006) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : SH-SY5Y cell lysate
      Lane 4 : NCI-H460 cell lysate
      Lane 5 : Jurkat cell lysate
      Lane 6 : JAR cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 58 kDa
    • ab125006 at 1/100 dilution staining LMAN1 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
    • ab125006 at 1/100 dilution staining LMAN1 in paraffin-embedded Human papillary carcinoma of thyroid gland tissue by Immunohistochemistry.

    Anti-LMAN1 antibody [EPR6979] (ab125006) 使用論文

    This product has been referenced in:
    • Duellman T  et al. LMAN1 (ERGIC-53) is a potential carrier protein for matrix metalloproteinase-9 glycoprotein secretion. Biochem Biophys Res Commun 464:685-91 (2015). ICC/IF ; Human . Read more (PubMed: 26150355) »

    See 1 Publication for this product

    Product Wall

    Thank you for contacting us. The specific immunogen sequences for these antibodies are proprietary. They are both internal sequences, but do not overlap with amino acids 88 - 181.
    The lab has tested HeLa and JAR cells in immunocytochemistry, but u...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"