Anti-LMAN1 抗体 (ab32583)
Key features and details
- Rabbit polyclonal to LMAN1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-LMAN1 antibody
LMAN1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to LMAN1 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide:
FDSFDNDGKKNNPAI
, corresponding to amino acids 159-173 of Rat LMAN1 -
ポジティブ・コントロール
- ICC: p19 cells, HMVEC cells, A549 cells. WB: Rat brain lysate. HepG2, HeLa, PANC-1, and MCF7 lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32583の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use at an assay dependent concentration.
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WB |
1/1000. Detects a band of approximately 57.5 kDa.
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特記事項 |
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ICC/IF
Use at an assay dependent concentration. |
WB
1/1000. Detects a band of approximately 57.5 kDa. |
ターゲット情報
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機能
Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins. -
組織特異性
Ubiquitous. -
関連疾患
Defects in LMAN1 are THE cause of factor V and factor VIII combined deficiency type 1 (F5F8D1) [MIM:227300]; also known as multiple coagulation factor deficiency I (MCFD1). F5F8D1 is an autosomal recessive blood coagulation disorder characterized by bleeding symptoms similar to those in hemophilia or parahemophilia, that are caused by single deficiency of FV or FVIII, respectively. The most common symptoms are epistaxis, menorrhagia, and excessive bleeding during or after trauma. Plasma levels of coagulation factors V and VIII are in the range of 5 to 30% of normal. -
配列類似性
Contains 1 L-type lectin-like domain. -
翻訳後修飾
The N-terminal may be partly blocked. -
細胞内局在
Endoplasmic reticulum-Golgi intermediate compartment membrane. Golgi apparatus membrane. Endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3998 Human
- Entrez Gene: 70361 Mouse
- Entrez Gene: 116666 Rat
- Omim: 601567 Human
- SwissProt: P49257 Human
- SwissProt: Q9D0F3 Mouse
- SwissProt: Q62902 Rat
- Unigene: 465295 Human
see all -
別名
- Endoplasmic reticulum golgi intermediate compartment protein 53 antibody
- ER-Golgi intermediate compartment 53 kDa protein antibody
- ERGIC-53 antibody
see all
画像
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ab32583 labelling LMAN1 in p19 cells by Immunocytochemistry/Immunofluorescence.
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ab32583 labelling LMAN1 in HMVEC cells by Immunocytochemistry/Immunofluorescence.
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All lanes : Anti-LMAN1 antibody (ab32583) at 1/2000 dilution
Lane 1 : HepG2 lysate
Lane 2 : HeLa lysate
Lane 3 : PANC-1 lysate
Lane 4 : MCF7 lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/4000 dilution -
ab32583 labelling LMAN1 in A549 cells by Immunocytochemistry/Immunofluorescence.
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All lanes : Anti-LMAN1 antibody (ab32583) at 1/1000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat brain lysate with Immunising peptide
Observed band size: 57.5 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab32583 は論文での使用が確認できていません。