製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to LLGL1
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WBmore details
  • 種交差性

    交差が予測される動物種: Mouse, Rat, Cow, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Lgl1.

    (Peptide available as ab39291.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following tissue lysates; Human brain and Mouse brain.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab39292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 115 kDa).

ターゲット情報

  • 機能
    Cortical cytoskeleton protein found in a complex involved in maintaining cell polarity and epithelial integrity. Involved in the regulation of mitotic spindle orientation, proliferation, differentiation and tissue organization of neuroepithelial cells.
  • 組織特異性
    Expressed in brain, kidney, and muscle but is barely seen in heart and placenta. Down-regulated or lost in all cell lines and in most of the tumor samples analyzed. Loss was associated with advanced stage of the disease.
  • 配列類似性
    Belongs to the WD repeat L(2)GL family.
    Contains 14 WD repeats.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity). Phosphorylated at least at Ser-663 by PRKCI.
  • 細胞内局在
    Cytoplasm > cytoskeleton. Localized to the lateral membrane during the polarization and formation cell-cell contacts.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DLG4 antibody
    • DLG4, formerly antibody
    • Hugl 1 antibody
    • HUGL antibody
    • Hugl-1 antibody
    • HUGL1 antibody
    • Human homolog to the D-lgl gene protein antibody
    • L2GL1_HUMAN antibody
    • Lethal giant larvae homolog 1 (Drosophila) antibody
    • Lethal giant larvae homolog 1 antibody
    • Lethal(2) giant larvae protein homolog 1 antibody
    • LLGL antibody
    • Llgl1 antibody
    • Ly 49G2 antibody
    • Ly49G2 antibody
    • Mgl1 antibody
    • Mlgl antibody
    see all

画像

  • All lanes : Anti-LLGL1 antibody (ab39292) at 1 µg

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size: 115 kDa
    Observed band size: 125 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 26 kDa, 30 kDa, 50 kDa. We are unsure as to the identity of these extra bands.



    Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

プロトコール

参考文献

This product has been referenced in:
  • Song J  et al. Hugl-1 induces apoptosis in esophageal carcinoma cells both in vitro and in vivo. World J Gastroenterol 19:4127-36 (2013). WB ; Human . Read more (PubMed: 23864775) »

See 1 Publication for this product

レビューと Q&A

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (various: incl. SW620(+) HEK293 Ms Brain)
Loading amount
30 µg
Specification
various: incl. SW620(+) HEK293 Ms Brain
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

投稿 Nov 14 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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