製品の概要

  • 製品名Anti-LKB1 (phospho S428) antibody
    LKB1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to LKB1 (phospho S428)
  • 特異性ab63473 detects endogenous levels of LKB1 only when phosphorylated at serine 428.
  • アプリケーション適用あり: ELISA, ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic phosphopeptide derived from human LKB1 around the phosphorylation site of serine 428 (R-L-SP-A-C).

  • ポジティブ・コントロール
    • WB: Mouse brain extracts. ICC/IF: NIH3T3 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab63473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/40000.
ICC/IF 1/500 - 1/1000.
WB 1/500 - 1/1000. Predicted molecular weight: 48 kDa.

ターゲット情報

  • 機能Essential role in G1 cell cycle arrest. Phosphorylates and activates members of the AMPK-related subfamily of protein kinases. Tumor suppressor.
  • 組織特異性Ubiquitously expressed. Strongest expression in testis and fetal liver.
  • 関連疾患Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) [MIM:175200]. PJS is a rare hereditary disease in which there is predisposition to benign and malignant tumors of many organ systems. PJS is an autosomal dominant disorder characterized by melanocytic macules of the lips, multiple gastrointestinal hamartomatous polyps and an increased risk for various neoplasms, including gastrointestinal cancer.
    Defects in STK11 have been associated with testicular tumors (TEST) [MIM:273300]. A common solid malignancy in males. Germ cell tumors of the testis constitute 95% of all testicular neoplasms.
  • 配列類似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. LKB1 subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Phosphorylated by a cAMP-dependent protein kinase.
  • 細胞内局在Nucleus. Cytoplasm. Relocates to the cytoplasm when bound to CAB39 and STRAD or CAB39 and ALS2CR2.
  • Information by UniProt
  • 参照データベース
  • 別名
    • hLKB1 antibody
    • Liver kinase B1 antibody
    • LKB1 antibody
    • PJS antibody
    • Polarization related protein LKB1 antibody
    • Renal carcinoma antigen NY-REN-19 antibody
    • Serine/Threonine Kinase 11 antibody
    • Serine/threonine protein kinase 11 antibody
    • Serine/threonine protein kinase LKB1 antibody
    • Serine/threonine protein kinase STK11 antibody
    • Serine/threonine-protein kinase 11 antibody
    • Serine/threonine-protein kinase LKB1 antibody
    • Serine/threonine-protein kinase XEEK1 antibody
    • Stk11 antibody
    • STK11_HUMAN antibody
    see all

Anti-LKB1 (phospho S428) antibody 画像

  • All lanes : Anti-LKB1 (phospho S428) antibody (ab63473) at 1/500 dilution

    Lane 1 : mouse brain extracts
    Lane 2 : mouse brain extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 48 kDa
    Observed band size : 48 kDa
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
  • ab63473 at 1/500 dilution staining LKB1 in NIH3T3 cells by Immunofluorescence, in the absence or presence of the immunising peptide.

Anti-LKB1 (phospho S428) antibody (ab63473) 使用論文

This product has been referenced in:
  • Konno H  et al. Cyclic Dinucleotides Trigger ULK1 (ATG1) Phosphorylation of STING to Prevent Sustained Innate Immune Signaling. Cell 155:688-98 (2013). Read more (PubMed: 24119841) »

See 1 Publication for this product

Product Wall

Thank you for contacting us.

The link to the database entry for the human LKB1 protein is:

http://www.uniprot.org/uniprot/Q15831

I hope this information is helpful to you. Please do not hesitate to contact us if you need...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Mouse brain)
Loading amount 50 µg
Specification Mouse brain
Gel Running Conditions Reduced Denaturing (10-20% tris-tricine)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Miss. Janine Mett

Verified customer

投稿 May 12 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"