製品の概要

  • 製品名Anti-Liver Carboxylesterase 1 antibody
    Liver Carboxylesterase 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Liver Carboxylesterase 1
  • アプリケーション適用あり: WB, IP, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Baboon, Rhesus monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human Liver Carboxylesterase 1.

    (Peptide available as ab66527.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following Lysates: HepG2 Whole Cell THP1 Whole Cell Mouse Liver Tissue Rat Liver Tissue This antibody gave a positive result in IHC in the following FFPE tissue: Human normal liver.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab45957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
IP Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate.
  • 組織特異性Expressed predominantly in liver with lower levels in heart and lung.
  • 配列類似性Belongs to the type-B carboxylesterase/lipase family.
  • 翻訳後修飾Contains sialic acid.
    Cleavage of the signal sequence can occur at 2 positions, either between Trp-17 and Gly-18 or between Gly-18 and His-19.
  • 細胞内局在Endoplasmic reticulum lumen.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ACAT antibody
    • Acyl coenzyme A cholesterol acyltransferase antibody
    • Acyl-coenzyme A:cholesterol acyltransferase antibody
    • Brain carboxylesterase hBr1 antibody
    • Carboxyesterase ES-3 antibody
    • Carboxylesterase antibody
    • Carboxylesterase 1 (monocyte/macrophage serine esterase 1) antibody
    • Carboxylesterase 1 antibody
    • Carboxylesterase 1 deficiency, included antibody
    • Carboxylesterase 2, formerly antibody
    • CE 1 antibody
    • CEH antibody
    • Ces-1 antibody
    • CES1 antibody
    • CES2 antibody
    • CESDD1 antibody
    • Cholesterol ester hydrolase, neutral, macrophage-derived antibody
    • Cholesteryl ester hydrolase antibody
    • Cocaine carboxylesterase antibody
    • EC 3.1.1.1 antibody
    • Egasyn antibody
    • ES-HTEL antibody
    • ES-x antibody
    • Es22 antibody
    • EST1_HUMAN antibody
    • Esterase 22 antibody
    • Esterase antibody
    • hCE 1 antibody
    • HMSE antibody
    • HMSE1 antibody
    • Liver carboxylesterase 1 antibody
    • Liver carboxylesterase 3 antibody
    • Methylumbelliferyl acetate deacetylase 1 antibody
    • MGC117365 antibody
    • MGC156521 antibody
    • Monocyte carboxylesterase deficiency, included antibody
    • Monocyte esterase deficiency, included antibody
    • Monocyte/macrophage serine esterase antibody
    • PCE-1 antibody
    • pI 5.5 esterase antibody
    • Proline-beta-naphthylamidase antibody
    • REH antibody
    • Retinyl ester hydrolase antibody
    • Serine esterase 1 antibody
    • Ses-1 antibody
    • SES1 antibody
    • TGH antibody
    • Triacylglycerol hydrolase antibody
    see all

Anti-Liver Carboxylesterase 1 antibody 画像

  • All lanes : Anti-Liver Carboxylesterase 1 antibody (ab45957) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : THP1 whole cell lysate (ab7913)
    Lane 3 : Liver (Mouse) Tissue Lysate - normal tissue
    Lane 4 : Liver (Rat) Tissue Lysate - normal tissue

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 63 kDa
    Observed band size : 63 kDa
  • ICC/IF image of ab45957 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45957, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 100% Methanol fixed (5 min) HepG2 cells at 1µg/ml.
  • IHC image of Carboxylesterase 1 staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45957, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Liver Carboxylesterase 1 was immunoprecipitated using 0.5mg Mouse Liver tissue lysate, 5µg of Rabbit polyclonal to Liver Carboxylesterase 1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab45957.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 63ka; Liver Carboxylesterase 1

Anti-Liver Carboxylesterase 1 antibody (ab45957) 使用論文

This product has been referenced in:
  • Xu J  et al. Hepatic carboxylesterase 1 is induced by glucose and regulates postprandial glucose levels. PLoS One 9:e109663 (2014). WB ; Mouse . Read more (PubMed: 25285996) »

See 1 Publication for this product

Product Wall

Thank you for your reply.


Can you please provide an order number for your purchase of these antibodies? If you are contacting us within 6 months of purchase, I am happy to offer a replacement or credit per our Abpromise guarantee.
Read More

Thank you for your reply with this protocol information.


I am sorry that you have been experiencing difficulties with two of our antibodies in WB. I have reviewed the protocol information you provided and I would like to ask some addition...

Read More

Thank you for your message which has been forwarded to me as my colleague is currently away from the office.  I am sorry to hear you have had some difficulty detecting the recombinant protein. I can confirm that  often the presence of a flag tag can...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"