製品の概要

  • 製品名Anti-liver Arginase antibody
    Liver Arginase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to liver Arginase
  • アプリケーション適用あり: ICC/IF, WB, IHC-P, IPmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: Pig
  • 免疫原

    Synthetic peptide corresponding to Mouse Liver Arginase aa 300 to the C-terminus (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    (Peptide available as ab100829, ab100829)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in mouse and rat liver tissue lysates.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab91279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa).
IHC-P Use at an assay dependent concentration.
IP Use a concentration of 5 µg/ml.

ターゲット情報

  • パスウェイNitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.
  • 関連疾患Defects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia.
  • 配列類似性Belongs to the arginase family.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A I antibody
    • Al antibody
    • ARG 1 antibody
    • arg1 antibody
    • ARGI1_HUMAN antibody
    • Arginase 1 antibody
    • Arginase liver antibody
    • Arginase type I antibody
    • Arginase, liver antibody
    • Arginase-1 antibody
    • Arginase1 antibody
    • Liver type arginase antibody
    • Liver-type arginase antibody
    • Type I arginase antibody
    see all

Anti-liver Arginase antibody 画像

  • All lanes : Anti-liver Arginase antibody (ab91279) at 1 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Observed band size : 37,40 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
  • Liver Arginase was immunoprecipitated using 0.5mg Mouse Liver tissue lysate, 5µg of Rabbit polyclonal to Liver Arginase and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab91279.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 37, 40kDa: liver Arginase
  • ICC/IF image of ab91279 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab91279, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-liver Arginase antibody (ab91279) 使用論文

This product has been referenced in:
  • Ballantyne LL  et al. Liver-specific knockout of arginase-1 leads to a profound phenotype similar to inducible whole body arginase-1 deficiency. Mol Genet Metab Rep 9:54-60 (2016). IHC-P, IF, WB ; Mouse . Read more (PubMed: 27761413) »
  • Sin YY  et al. Inducible arginase 1 deficiency in mice leads to hyperargininemia and altered amino acid metabolism. PLoS One 8:e80001 (2013). WB ; Mouse . Read more (PubMed: 24224027) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain)
Antigen retrieval step None
Permeabilization No
Specification Brain
Blocking step 10% donkey serum 3% BSA 0.3% triton-x100 as blocking agent for 2 hour(s) and 0 minute(s) · Temperature: 22°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Feb 18 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 25% · Temperature: 23°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Sample Mouse Tissue sections (Lung)
Specification Lung
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Oct 14 2014

DISCOUNT CODE: ***
Expiration date: September 1st, 2012

I am very pleased to hear you would like to accept our offer and test ab64693 in mouse. This code will give you: 1 freeprimary antibodybefore the expiration date. To redeem this off...

Read More

The current lot of ab91279, and any lots of at least 1mg/ml, will not contain BSA but will contain 0.02% Sodium azide. I hope this is helpful. Please contact me again if you need any further information.

Sorry for the late reply. I am still enquirying as to the availability of these 2 antibodies in BSA-free and azide-free formulations. Thanks for your patience!

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"