Application
Western blot
Sample
Mouse Cell lysate - whole cell (Primary Hepatocytes)
Loading amount
25 µg
Specification
Primary Hepatocytes
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Odyssey buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
45 minute(s) · Temperature: 25°C · Diluent: Odyssey buffer
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-mouse IgG-Alexa Fluor 680
Host species: Goat
Clonality: Polyclonal
Conjugation: HiLyte Plus™ 647
Host species: Goat
Clonality: Polyclonal
Conjugation: HiLyte Plus™ 647
Detection
Detection method
Odyssey imager
Exposure
4 minute(s) and 0 second(s)
Bands
Specific: 10-15 kDa
Positive control
Tubulin not shown in the image
Additional data
Additional Notes
Secondary antibody is Alexa Fluor 680 goat anti-mouse IgG (H+L)
LFABP is around 10-15kDa, the single band in WB.
LFABP is around 10-15kDa, the single band in WB.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 25 2007