Application
Immunocytochemistry/ Immunofluorescence
Sample
African Green Monkey Cell (hepatocyte)
Specification
hepatocyte
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Other product details
Dilution
1/50
Incubation time
45 minute(s) · Temperature: 25°C
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-mouse FITC
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Dilution
1/400
Additional data
Additional Notes
The staining was too weak to conclude
Abcam response
Thank you for your valuable feedback. We are sorry to hear that this antibody has not given you good results in Immunocytochemistry (ICC/IF). This antibody has not been tested in this application before, and it is possible that it is not suitable for use, or the vial you received may have been damaged in some way. However, we would be unable to confirm this without further testing of the antibody in ICC/IF application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 May 29 2008