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ab115347 is a one-step assay to differentially labels live and dead cells with fluorescent dyes. It is useful for the rapid quantitation of cell viability using flow cytometry or fluorescent microscopy. The provided Live and Dead Assay stain is sufficient for ~1000 assays.
The Live and Dead assay stain solution is a mixture of two highly fluorescent dyes that differentially label live and dead cells:
The Live cell dye labels intact, viable cells green. It is membrane permeant and non-fluorescent until ubiquitous intracellular esterases remove ester groups and render the molecule fluorescent. The Excitation (max) and Emission(max) are 494nm and 515nm, respectively (similar to FITC).
The Dead cell dye labels cells with compromised plasma membranes red. It is membrane-impermeant and binds to DNA with high affinity. Once bound to DNA, the fluorescence increases >30-fold. The Excitation (max) and Emission(max) are 528nm and 617nm, respectively.
The Live and Dead Cell Assay is a one-step staining procedure that is simple and fast. It can be used directly in cell culture media. Stained cells are not compatible with fixation.
Store the Live and Dead staining solution at -20°C. Allow the product to warm to room temperature before opening. Use diluted solutions of Live/Dead stain promptly.
|1000X Live/Dead Cell stain in DMSO||1 x 0.1ml|
Our Abpromise guarantee covers the use of ab115347 in the following tested applications.
|Functional Studies||Use at an assay dependent concentration.|
Dot plots showing live/dead analysis of vehicle or drug treated Jurkat cells (day 3 of treatment). The indicated drug is used to induce cell death. Live cells are on the y-axis and dead cells are on the x-axis. The red polygongate identifies live cells and the number indicates the percent of live cells.
Quantification of % viable cells of Jurkat cells treated with a dose response of the inidicated drug (to induce cell death) and analyzed using the live/dead assay stain on days 1, 2 and 3 using flow cytometry.
The sample dot plots demonstrate varying ratios of live and dead cells. More green = upper left = live cells; more red = lower right = dead cells.
Jurkat cells stained with the live/dead assay kit. Jurkat cells treated with a drug to induce cell death were labeled with the live/dead assay stain. Live cells (with esterase activity) stain green and dead cells (compromised plasma membrane) stain red. (A) Field of cells following 10 minute staining in media of live/dead stain. (B)Magnified view showing that in live cells the whole cell is stained green whereas in dead red cells it is the fragmented nuclear DNA that is stained.