Anti-Listeria monocytogenes 抗体 [LZH1] (ab11439)

製品の概要

  • 製品名Anti-Listeria monocytogenes antibody [LZH1]
    Listeria monocytogenes 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [LZH1] to Listeria monocytogenes
  • 特異性Reacts with L. Monocytogenes outer membrane fraction and intact cells.
  • アプリケーション適用あり: ELISA, WB, Sandwich ELISAmore details
  • 種交差性
    This antibody does not react with the following species: E. coli, Salmonella, Y. pestis, F. tularensis, L. pneumophila and B. abortus. Ab11439 does react with L. innocua and L. ivanovii.
  • 免疫原

    Listeria monocytogenes.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)Purity tested by electrophoresis.
  • ポリ/モノモノクローナル
  • クローン名LZH1
  • ミエローマSp2/0
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab11439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use a concentration of 0.001 - 3 µg/ml. Coat with L. monocytogenes outer membrane fraction, 5 ug/ml in 0.1 M carbonate buffer, pH 9.2, overnight at + 4 °C. Add Mouse monoclonal [LZA2] to Listeria monocytogenes in PBST, 1 h, 37 °C.
WB Use a concentration of 8 µg/ml. Predicted molecular weight: 23 kDa. Use 15 % SDS-PAGE electrophoresis in reducing conditions (30 ug antigen per track) and transfer to nitrocellulose membrane (60 min, 100 mA). Blocking: 1 % BSA in PBS, 1 h, 37 °C.
Sandwich ELISA Use a concentration of 0.001 - 3 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [LZF7] to Listeria monocytogenes (HRP) (ab24456). Use this Capture antibody at a concentration of 1ng/ml - 3 µg/ml.

ターゲット情報

  • 関連性The genus Listeria comprises six species: L. monocytogenes, L. innocua, L. welshimeri, L. seeligeri, L. ivanovii and L. grayi. Listeria monocytogenes, the most commonly isolated pathogenic member, is associated with a wide spectrum of human and animal diseases. In the smear from the original tissue, L. monocytogenes may appear as gram-positive coccobacilli that may be confused with Streptococcus agalactiae (group B), enterococci, or Corynebacterium spp. Listeria is differentiated from streptococci by a positive catalase test. L. monocytogenes is the only species of the genus Listeria that has been clearly documented as a pathogen for humans. The forms of disease caused by this organism are myriad and age-related. The most common clinical manifestations are meningitis and septicemia. Listeria monocytogenes, a food-borne intracellular animal and human pathogen, interacts with infected host cells both prior to entry and during the intracellular phase of infection.
  • 別名
    • L. monocytogenes antibody

Anti-Listeria monocytogenes antibody [LZH1] (ab11439) 使用論文

ab11439 has not yet been referenced specifically in any publications.

Product Wall

Antibody ab11439 (LZH1 clone) was used for capture and ab24456 for detection. The antibodies were tested at various dilutions.

ab11439: Use a concentration of 0.001 - 3 µg/ml.

ab24456: Use a concentration of 1ng/ml - 3 µg/ml.
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We have a report that these antibodies are working in ELISA and WB at least with heat-killed Listeria monocytogenes cells from KPL Inc. (Cat.# 50-90-90).

Thank you for contacting us.

I have been informed by the source of the antibody ab11439 that the outer membrane fraction mentioned on the datasheet is a complex antigen of the Listeria monocytogenes cell membrane. Sorry about the confusing nam...

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Thank you for your enquiry. To help answer your customer's question, could you please let me know what specific application they will use the antibodies for (eg. western blotting, IHC, etc). Thanks!

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Listeria monocytogenes Cell (Bacteria)
Specification Bacteria
Fixative Paraformaldehyde
Permeabilization Yes - Triton-X 0.5%
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Abcam user community

Verified customer

投稿 Aug 31 2011

Thank you for your enquiry, we do not supply a positive control with this Ab; however I am in contact with the originator in order to confirm what was used as a positive control in ELISA during testing. I will be in touch again when I have a response. ...

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I enclose the protocols that were used to test these antibodies: Direct ELISA: Use at a concentration of 1ng/ml - 3 µg/ml. Coat with L. monocytogenes outer membrane fraction, 5 ug/ml in 0.1 M carbonate buffer, pH 9.2, overnight at +4°C. Add ab...

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Thank you for your enquiry regarding our Salmonella typhimurium, Listeria monocytogenes, or Campylobacter jejuni antibodies. For Listeria monocytogenes detection we recommend pair LZH1 (ab11439) – LZF7 (ab11440)(capture – conjugate) for sandwich imm...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"