Anti-Listeria monocytogenes 抗体 [LZA2] (ab11438)

製品の概要

  • 製品名Anti-Listeria monocytogenes antibody [LZA2]
    Listeria monocytogenes 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [LZA2] to Listeria monocytogenes
  • 特異性Recognises L. Monocytogenes outer membrane fraction and whole cells.
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    This antibody does not react with the following species: E. coli, Salmonella, Y. pestis, F. tularensis, L. pneumophila and B. abortus.
  • 免疫原

    Listeria monocytogenes.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度Purified IgM
  • 特記事項(精製)Clone LZA2 is purified by euglobulin precipitation. Purity tested by electrophoresis.
  • ポリ/モノモノクローナル
  • クローン名LZA2
  • ミエローマSp2/0
  • アイソタイプIgM
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab11438 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
  • 追加情報Direct ELISA: Use at a concentration of 1ng/ml - 3 µg/ml.
    Coat with L. monocytogenes outer membrane fraction, 5 ug/ml in 0.1 M carbonate buffer, pH 9.2, overnight at + 4 °C. Add ab11438 in PBST, 1 h, 37 °C.
    Use anti-mouse IgG HRP-conjugated in PBST 1 hr, 37 °C. Substrate: TMB, 30 min.

    WB: Use at a concentration of 8 µg/ml. Use 15 % SDS-PAGE electrophoresis in reducing conditions (30 ?g antigen per track) and transfer to nitrocellulose membrane (60 min, 100 mA). Blocking: 1 % BSA in PBS, 1 h, 37 °C.
    Detects a band of approximately 23 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性The genus Listeria comprises six species: L. monocytogenes, L. innocua, L. welshimeri, L. seeligeri, L. ivanovii and L. grayi. Listeria monocytogenes, the most commonly isolated pathogenic member, is associated with a wide spectrum of human and animal diseases. In the smear from the original tissue, L. monocytogenes may appear as gram-positive coccobacilli that may be confused with Streptococcus agalactiae (group B), enterococci, or Corynebacterium spp. Listeria is differentiated from streptococci by a positive catalase test. L. monocytogenes is the only species of the genus Listeria that has been clearly documented as a pathogen for humans. The forms of disease caused by this organism are myriad and age-related. The most common clinical manifestations are meningitis and septicemia. Listeria monocytogenes, a food-borne intracellular animal and human pathogen, interacts with infected host cells both prior to entry and during the intracellular phase of infection.
    • 別名
      • L. monocytogenes antibody

    Anti-Listeria monocytogenes antibody [LZA2] (ab11438) 使用論文

    ab11438 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry. To help answer your customer's question, could you please let me know what specific application they will use the antibodies for (eg. western blotting, IHC, etc). Thanks!

    Thank you for your enquiry. The concentration of this lot of the antibody is 4.40 mg/ml. The size we sell is 100ug, but it appears that you actually received nearly double that amount! I hope this information helps. Please do not hesitate to c...

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    I enclose the protocols that were used to test these antibodies: Direct ELISA: Use at a concentration of 1ng/ml - 3 µg/ml. Coat with L. monocytogenes outer membrane fraction, 5 ug/ml in 0.1 M carbonate buffer, pH 9.2, overnight at +4°C. Add ab...

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    Thank you for your enquiry regarding our Salmonella typhimurium, Listeria monocytogenes, or Campylobacter jejuni antibodies. For Listeria monocytogenes detection we recommend pair LZH1 (ab11439) – LZF7 (ab11440)(capture – conjugate) for sandwich imm...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"