製品の概要

  • 製品名Anti-LIS1 antibody
    LIS1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to LIS1
  • アプリケーション適用あり: WB, IHC-P, ICC/IF, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • 免疫原

    Synthetic peptide corresponding to Human LIS1. Represents a portion of human Lissencephaly 1 (Lis 1) protein encoded in part by exons 3 and 4.

  • ポジティブ・コントロール
    • TF-1 (erythroleukemia cell line) cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 特記事項(精製)Affinity purified using the immunising peptideimmobilized on solid support.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab2607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-P
ICC/IF
IP
  • 追加情報ICC/IF: Use at an assay dependent dilution (see PMID: 17314247).
    IP: Use at a concentration of 2 - 10 µg/ml.
    WB: 1/300 - 1/3000. Suggested working dilution of 1/1000 (see PMID: 17314247).
    IHC-P: Use at a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Required for proper activation of Rho GTPases and actin polymerization at the leading edge of locomoting cerebellar neurons and postmigratory hippocampal neurons in response to calcium influx triggered via NMDA receptors. Non-catalytic subunit of an acetylhydrolase complex which inactivates platelet-activating factor (PAF) by removing the acetyl group at the SN-2 position (By similarity). Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. Required during brain development for the proliferation of neuronal precursors and the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Neuronal migration involves a process called nucleokinesis, whereby migrating cells extend an anterior process into which the nucleus subsequently translocates. During nucleokinesis dynein at the nuclear surface may translocate the nucleus towards the centrosome by exerting force on centrosomal microtubules. May also play a role in other forms of cell locomotion including the migration of fibroblasts during wound healing.
    • 組織特異性Fairly ubiquitous expression in both the frontal and occipital areas of the brain.
    • 関連疾患Defects in PAFAH1B1 are the cause of lissencephaly type 1 (LIS1) [MIM:607432]; also known as classic lissencephaly. LIS1 is characterized by agyria or pachgyria and disorganization of the clear neuronal lamination of normal six-layered cortex. The cortex is abnormally thick and poorly organized with 4 primitive layers. LIS1 is associated with enlarged and dysmorphic ventricles and often hypoplasia of the corpus callosum.
      Defects in PAFAH1B1 are the cause of subcortical band heterotopia (SBH) [MIM:607432]. SBH is a mild brain malformation of the lissencephaly spectrum. It is characterized by bilateral and symmetric ribbons of gray matter found in the central white matter between the cortex and the ventricular surface.
      Defects in PAFAH1B1 are a cause of Miller-Dieker lissencephaly syndrome (MDLS) [MIM:247200]. MDLS is a contiguous gene deletion syndrome of chromosome 17p13.3, characterized by classical lissencephaly and distinct facial features. Additional congenital malformations can be part of the condition.
    • 配列類似性Belongs to the WD repeat LIS1/nudF family.
      Contains 1 LisH domain.
      Contains 7 WD repeats.
    • ドメインDimerization mediated by the LisH domain may be required to activate dynein.
    • 細胞内局在Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Nucleus membrane. Redistributes to axons during neuronal development. Also localizes to the microtubules of the manchette in elongating spermatids and to the meiotic spindle in spermatocytes (By similarity). Localizes to the plus end of microtubules and to the centrosome. May localize to the nuclear membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • LIS 1 antibody
      • LIS 2 antibody
      • LIS-1 antibody
      • LIS1 antibody
      • LIS1_HUMAN antibody
      • LIS2 antibody
      • Lissencephaly 1 protein antibody
      • Lissencephaly-1 protein antibody
      • MDCR antibody
      • MDS antibody
      • PAF acetylhydrolase 45 kDa subunit antibody
      • PAF AH 45 kDa subunit antibody
      • PAF AH alpha antibody
      • PAF-AH 45 kDa subunit antibody
      • PAF-AH alpha antibody
      • PAFAH alpha antibody
      • PAFAH antibody
      • PAFAH1B1 antibody
      • PAFAHA antibody
      • Platelet activating factor acetylhydrolase 1b regulatory subunit 1 antibody
      • Platelet activating factor acetylhydrolase isoform Ib alpha subunit antibody
      • Platelet-activating factor acetylhydrolase IB subunit alpha antibody
      see all

    Anti-LIS1 antibody 画像

    • ab2607 (2µg/ml) staining Lis1 in human testis using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cytoplasmic compartment of the mature spermatocyte cells.
      Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

    Anti-LIS1 antibody (ab2607) 使用論文

    This product has been referenced in:
    • Sudarov A  et al. Lis1 controls dynamics of neuronal filopodia and spines to impact synaptogenesis and social behaviour. EMBO Mol Med 5:591-607 (2013). IHC-P ; Mouse . Read more (PubMed: 23483716) »
    • Shyamasundar S  et al. Analysis of epigenetic factors in mouse embryonic neural stem cells exposed to hyperglycemia. PLoS One 8:e65945 (2013). WB ; Mouse . Read more (PubMed: 23776576) »

    See all 12 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Embryo)
    Permeabilization Yes - 0,5%TritonX + 0,05%Tween20
    Specification Embryo
    Blocking step Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Fixative Paraformaldehyde
    Username

    Lynn Vandenberghe

    Verified customer

    投稿 Aug 17 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Caco-2 cell)
    Specification Caco-2 cell
    Fixative Methanol
    Permeabilization No
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    投稿 Mar 28 2013

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    We will not send you a sample of either a new or old lot of the ab2607 as we are not able to provi...

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    Thank you for your reply.

    DISCOUNT CODE: xxx
    Expiration date: xxx

    I am very pleased to hear you would like to accept our offer and test ab2607 in IHC-whole mount. This code will give you: 1 free PRIMARY ANTIBODY before the exp...

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    Will the mouse tissue be paraffin embedded or fresh frozen, or neither of them?

    Could you please let me know? Thank you.


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    Thank you for contacting Abcam. I have looked over all the details that you provided and I would recommended that you increase you incubation of the primary antibody to an overnight incubation at 4C with gently agitation. If increasing the incubation t...

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    Thank you for your patience; I was away on vacation and could not reply to you earlier. As you previously used a different lot of ab2607 successfully under the same conditions, my hunch is that the latest vial that you received somehow went off. Pl...

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