Anti-Lipoprotein lipase 抗体 [LPL.A4] (ab21356)

製品の概要

  • 製品名
    Anti-Lipoprotein lipase antibody [LPL.A4]
    Lipoprotein lipase 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [LPL.A4] to Lipoprotein lipase
  • アプリケーション
    適用あり: ICC/IF, IHC-P, Flow Cyt, WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Cow, Human
  • 免疫原

    Full length protein (Human).

  • ポジティブ・コントロール
    • Purified human and bovine recombinant Lipoprotein lipase.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab21356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 21062953
IHC-P Use at an assay dependent concentration. PubMed: 20200316
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB 1/1000. Predicted molecular weight: 53 kDa.
ELISA 1/2000 - 1/5000.

ターゲット情報

  • 機能
    The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium.
  • 関連疾患
    Defects in LPL are the cause of lipoprotein lipase deficiency (LPL deficiency) [MIM:238600]; also known as familial chylomicronemia or hyperlipoproteinemia type I. LPL deficiency chylomicronemia is a recessive disorder usually manifesting in childhood. On a normal diet, patients often present with abdominal pain, hepatosplenomegaly, lipemia retinalis, eruptive xanthomata, and massive hypertriglyceridemia, sometimes complicated with acute pancreatitis.
  • 配列類似性
    Belongs to the AB hydrolase superfamily. Lipase family.
    Contains 1 PLAT domain.
  • 翻訳後修飾
    Tyrosine nitration after lipopolysaccharide (LPS) challenge down-regulates the lipase activity.
  • 細胞内局在
    Cell membrane. Secreted. Locates to the plasma membrane of microvilli of hepatocytes with triacyl-glycerol-rich lipoproteins (TRL). Some of the bound LPL is then internalized and located inside non-coated endocytic vesicles.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EC 3.1.1 antibody
    • EC 3.1.1.34 antibody
    • HDLCQ11 antibody
    • LIPD antibody
    • LIPL_HUMAN antibody
    • Lipoprotein lipase antibody
    • LPL antibody
    • LPL protein antibody
    • MGC137861 antibody
    see all

Anti-Lipoprotein lipase antibody [LPL.A4] 画像

  • All lanes : Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356) at 1/1000 dilution

    Lane 1 : Human Lipoprotein lipase.
    Lane 2 : Bovine Lipoprotein lipase

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 53 kDa
    Observed band size : 55,60 kDa (why is the actual band size different from the predicted?)
  • ab21356 staining Lipoprotein lipase in Mouse small intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1.5% serum for 30 minutes at 23°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (2 ug/ml) for 14 hours at 4°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab21356 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab21356, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • ab21356 staining Lipoprotein lipase in Mouse intestine cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% BSA for 2 hours at 23°C. Samples were incubated with primary antibody (1/500) for 8 hours at 4°C. An Alexa Fluor® 488-conjugated Rat anti-mouse IgG1 polyclonal (1/250) was used as the secondary antibody.

    See Abreview

Anti-Lipoprotein lipase antibody [LPL.A4] (ab21356) 使用論文

This product has been referenced in:
  • Bal NC  et al. Increased Reliance on Muscle-based Thermogenesis upon Acute Minimization of Brown Adipose Tissue Function. J Biol Chem 291:17247-57 (2016). Read more (PubMed: 27298322) »
  • Alfaradhi MZ  et al. Oxidative stress and altered lipid homeostasis in the programming of offspring fatty liver by maternal obesity. Am J Physiol Regul Integr Comp Physiol 307:R26-34 (2014). WB ; Mouse . Read more (PubMed: 24789994) »

See all 17 Publications for this product

Product Wall

This antibody was raised against the whole Human LPL whole protein as stated in the datasheet.

Unfortunately, the exact epitope is unknown as the whole protein with all regions intact was used to produce the antibody and the epitope has not be...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Sample
Mouse Tissue lysate - whole (Liver)
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Sep 11 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1.5µg/mL · Temperature: 23°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citra Plus
Sample
Mouse Tissue sections (Small Intestine)
Specification
Small Intestine
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 28 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Loading amount
30 µg
Specification
HEK293
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Mr. Dongil Kim

Verified customer

投稿 Apr 11 2013

Thank you for your reply and for confirming those details.

Your credit note ID is ***.

I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you,...

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One more thing. Could you please tell me what dilution you've tried with this secondary antibody, for our records?

Thank you so much!

Thank you for your reply.

I have recorded all of the information so that we can further investigate the trouble with the antibody. I again apologize that it is not working as expecting, but I'm glad you've found a way to get some results. Read More

Thank you for your reply and for keeping me updated.

I am glad to hear that some bands were seen on the membrane, but I am sorry that it took such a high concentration in order to work in this assay. I have only been able to get the QC data f...

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Thank youagain for letting us know about the trouble with this antibody.

Have you had a chance to try the replacement vial of ab21356 from a new lot, or done any other testing?

I have received the QC data from the lab for the first ...

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Thank you so much for forwarding this data.

I will let you know once I havefurther informationfrom the lab. Please keep me updated about new results with the new vial.

I look forward to hearing from you and hope you're having a grea...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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