製品の概要
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製品名Anti-Lipoprotein lipase antibody
Lipoprotein lipase 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Lipoprotein lipase
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特異性No cross reactivity with other proteins is observed.
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アプリケーション適用あり: IHC-P, WB, ICC/IFmore details
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種交差性交差種: Cow, Human
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免疫原
A synthetic peptide corresponding to a sequence at the C-terminal of Human Lipoprotein lipase, different to the related rat sequence by two amino acids.
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ポジティブ･コントロール
- MCF7, HeLa, Jurkat and Smmc whole cell lystes. IHC-P: Human heart FFPE tissue sections. IF/ICC: HeLa cell line
法規制情報
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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バッファーPreservative: 0.02% Sodium Azide, 0.01% Thimerosal (merthiolate)
Constituents: 7mM Dibasic monohydrogen sodium phosphate, 5mg BSA, 0.9mg Sodium chloride -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab86927 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa. | |
| ICC/IF | Use a concentration of 5 µg/ml. |
ターゲット情報
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機能The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium.
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関連疾患Defects in LPL are the cause of lipoprotein lipase deficiency (LPL deficiency) [MIM:238600]; also known as familial chylomicronemia or hyperlipoproteinemia type I. LPL deficiency chylomicronemia is a recessive disorder usually manifesting in childhood. On a normal diet, patients often present with abdominal pain, hepatosplenomegaly, lipemia retinalis, eruptive xanthomata, and massive hypertriglyceridemia, sometimes complicated with acute pancreatitis.
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配列類似性Belongs to the AB hydrolase superfamily. Lipase family.
Contains 1 PLAT domain. -
翻訳後修飾Tyrosine nitration after lipopolysaccharide (LPS) challenge down-regulates the lipase activity.
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細胞内局在Cell membrane. Secreted. Locates to the plasma membrane of microvilli of hepatocytes with triacyl-glycerol-rich lipoproteins (TRL). Some of the bound LPL is then internalized and located inside non-coated endocytic vesicles.
- Information by UniProt
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参照データベース
- Entrez Gene: 280843 Cow
- Entrez Gene: 4023 Human
- Omim: 609708 Human
- SwissProt: P11151 Cow
- SwissProt: P06858 Human
- Unigene: 180878 Human
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別名
- EC 3.1.1 antibody
- EC 3.1.1.34 antibody
- HDLCQ11 antibody
see all
Anti-Lipoprotein lipase antibody 画像
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Immunocytochemistry/ Immunofluorescence - Anti-Lipoprotein lipase antibody (ab86927)ICC/IF image of ab86927 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86927, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lipoprotein lipase antibody (ab86927)IHC image of Lipoprotein lipase staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab86927, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Lipoprotein lipase antibody (ab86927)All lanes : Anti-Lipoprotein lipase antibody (ab86927) at 1 µg/ml
Lane 1 : Milk
Lane 2 : Milk
Lane 3 : MCF7 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lane 5 : Jurkat whole cell lysate
Lane 6 : Smmc whole cell lysate
Predicted band size : 53 kDa
Observed band size : 85 kDa (why is the actual band size different from the predicted?)
Additional bands at : <29 kDa. We are unsure as to the identity of these extra bands.N.B. There are two gylcosylation sites on the protein.
プロトコール
Anti-Lipoprotein lipase antibody (ab86927) 使用論文
ab86927 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"